海参磷脂型二十碳五烯酸对链脲佐菌素诱导的糖尿病大鼠肾脏功能改善作用的研究  

Protection of EPA-PC liposome on kidney of diabetic rats induced by STZ

作　　者：刘月娇[1] 徐雷雷[1] 柳东[1] 李冰[1] 李辉[1] 张珣[1] 王静凤[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2012年第19期331-334,共4页Science and Technology of Food Industry

基　　金：国家自然基金资助项目(30972284);海洋公益性行业科研专项资金资助项目(201105029);国际科技合作项目(2010DFA31330);浙江省重大科技专项(2008C02009);泰山学者建设工程专项经费

摘　　要：目的:研究海参磷脂型二十碳五烯酸(Eicosapentaenoic Acid Phosphatidy lcholine,EPA-PC)对链脲佐菌素(Streptozocin,STZ)诱导的糖尿病大鼠肾脏功能的改善作用。方法:通过腹腔注射STZ建立糖尿病大鼠模型,经不同剂量海参EPA-PC(25和75mg/kg,以EPA含量计)灌胃8w。实验结束后,分别检测大鼠空腹血糖、口服葡萄糖耐量、肾脏指数、24h尿量、血清尿素氮(BUN)、尿总蛋白、白蛋白(mAlb)、尿酸(UA)含量及β-N乙酰葡萄糖苷酶(NAG)活性。结果:海参EPA-PC可显著改善糖尿病大鼠的口服葡萄糖耐量(p<0.05),降低糖尿病大鼠的肾脏指数(p<0.01)、24h尿量、血清BUN、尿总蛋白、mAlb、UA含量及NAG活性(p<0.01)。结论:海参EPA-PC对糖尿病大鼠的肾脏功能具有较好的改善作用。Objective：To study the protective effects of Eicosapentaenoic Acid Phosphatidylcholine（EPA-PC）on kidney of diabetic rats induced by Streptozocin（STZ）.Methods：Diabetes mellitus induced by STZ were administered by different dosages of EPA-PC（25 mg/kg,75 mg/kg）by stomach perfusion,for 8 weeks.At the end of the experiment,the indices including the changes of the levels of fasting blood glucose,Oral glucose tolerance,the renal index,the 24h urine volume,the levels of BUN in plasma,the excretion of protein,mAlb,BUN,UA and NAG in urine were determined.Results：The EPA-PC showed remarkable hypoglycemic effected on Oral glucose tolerance of diabetic rats（p0.05）,and decreased the renal protein,mAlb,UA and NAG in urine had reduced significantly（p0.01）.Conclusion：EPA-PC can effectively improve the kidney function in diabetic rats.

关 键 词：多不饱和脂肪酸 海参EPA-PC 糖尿病肾病 血糖 尿白蛋白 肾脏保护 

分 类 号：R965[医药卫生—药理学]