重庆地区成人感染性腹泻病原学分析  

Etiological study of enteric viruses and bacteria in adult diarrhea in Chongqing area

作　　者：娄茜[1] 孙滨[1] 张再宽[1] 张秀瑜[1] 王云英[1] 黄长武[1] 陈维贤[1] 

机构地区：[1]重庆医科大学附属第二医院检验科,重庆400010

出　　处：《中国微生态学杂志》2012年第9期781-785,共5页Chinese Journal of Microecology

基　　金："十一五"科技重大专项"艾滋病和病毒性肝炎等重大传染病防治"-"传染病监测技术平台项目"(2009ZX10004)

摘　　要：目的研究重庆地区成人感染性腹泻患者的病原学特点。方法采用MICROSCAN系统进行粪便细菌培养,用ELISA和多重PCR方法进行病毒检测。结果 130例腹泻标本中,检出细菌阳性17例,包括沙门菌属7例,志贺菌属5例,副溶血弧菌3例和嗜水气单胞菌2例。病毒检测中,单重感染30例,双重感染10例,三重感染1例。A组轮状病毒检出率为3.85%(5/130),B轮状病毒检出率为3.85%(5/130),C组轮状病毒检出率为15.4%(20/130),诺如病毒检出率为9.23%(12/130),星状病毒检出率为4.62%(6/130),札如病毒检出率为3.08%(4/130),腺病毒检出率为0.77%(1/130)。结论重庆地区成人腹泻患者中,沙门菌和志贺菌为细菌感染的主要菌种,轮状病毒和诺如病毒为病毒感染的主要病原体。Objective To study the etiologic characteristics of enteric viruses and bacteria in adult diarrhea in Chongqing area.Methods Bacteria were isolated from feces specimens and indentified by MICROSCAN system.Viruses were detected with ELISA and multiple polymerase chain reactions（PCR）.Results A total of 17 strains of pathogenic bacteria were isolated from 130 specimens,including 7 strains of Salmonella,5 strains of Shigella,3 strains of vice Hemolysis vibrio and 2 strains of Aeromonas hydrophilia.Among the virus detection results,the positive rates for Rotavirus A,Rotavirus B,Rotavirus C,Norovirus,Astrovirus,Sapporo like virus and Adenovirus were 3.85%（5/130）,3.85%（5/130）,15.4%（20/130）,9.23%（12/130）,4.62%（6/130）,3.08%（4/130） and 0.77%（1/130）,respectively.Conclusion The prevalent pathogens for adult diarrhea in Chongqing area are Salmonella,Shigella,Rotavirus and Norovirus.

关 键 词：腹泻 细菌 病毒 ELISA 多重PCR 

分 类 号：R574.62[医药卫生—消化系统]