西宁地区居民鼻腔NK/T细胞淋巴瘤中细胞毒蛋白的表达及其与EB病毒感染的关系  

EXPRESSION OF CYTOTOXIC PROTEINS IN NASAL NK/T CELL LYMPHOMA AND ITS ASSOCIATION WITH EPSTIN BARR VIRUS AT XINING AND XI′AN AREAS

作　　者：冶俊玲[1] 韩静绮[1] 郭新建[1] 王丰梅[1] 

机构地区：[1]青海大学附属医院病理科

出　　处：《青海医学院学报》2012年第3期199-201,共3页Journal of Qinghai Medical College

摘　　要：目的探讨西宁地区居民鼻腔NK/T细胞淋巴瘤中细胞毒蛋白的表达及其与EB病毒(EBV)感染的关系。方法以西宁地区和西安地区鼻腔NK/T细胞淋巴瘤各55例石蜡标本为研究对象,同部位B细胞淋巴瘤20例为对照,采用免疫组织化学方法检测CD45RO、CD3、CD20、CD56、TIA-1确定肿瘤细胞的免疫表型。采用原位杂交技术检测EBV编码的mRNA(EBER)。结果西宁地区55例鼻腔NK/T细胞淋巴瘤中CD45RO阳性55例(100%),CD3阳性50例(90.9%),TIA-1阳性36例(65.5%),CD56阳性30例(54.5%),EBER阳性50例(90.9%);西安地区55例鼻腔NK/T细胞淋巴瘤中CD45RO阳性55例(100%),CD3阳性52例(94.5%),TIA-1阳性32例(58.2%),CD56阳性33例(60%),EBER阳性35例(63.6%);两地CD20均阴性。20例面部B细胞淋巴瘤中CD45RO、CD3、TIA-1、CD56均阴性,CD20均阳性,EBER阳性2例(10%)。结论西宁地区鼻腔NK/T细胞淋巴瘤中EBV感染率明显高于西安地区,提示EBV感染与西宁地区鼻腔NK/T细胞淋巴瘤的相关性更强。TIA-1对NK/T细胞淋巴瘤的标记有很好的敏感性和特异性,但无地区性。Objective To study the expression and significance of cytotoxic proteins in nasal NK/T cell lymphomas and its association with Epstin Barr Virus（EBV）at Xining area.Methods 55 cases of paraffin embedded tissues of nasal NK/T cell lymphoma in both Xining and Xi′ an areas for the study.20 midline B-cell lymphoma at same location as control was studied using immunohistochemical method.CD45RO,CD3,CD20,CD56 and TIA-1 were detected by immunohistochemical staining for investigate immunophenotype.EBV-encoded RNA（EBER）were detected by in situ hybridization（ISH）.Results Among the 55 cases in Xining,CD45RO was positive in all the patients（100%）;CD3 was positive in 50 cases（90.9%）;TIA-1 was positive in 36 cases（65.5%）;CD56 was positive in 30 cases（54.5%）and EBER was positive in 50 cases（90.9%）.Among the 55 cases in Xi′an,CD45RO was positive in all the 55 cases（100%）;CD3 was positive in 52 cases（94.5%）;TIA-1 was positive in 32 cases（58.2%）;CD56 was positive in 33 cases（60%）and EBER was positive in 35 cases（63.6%）.while CD20 negative in 55 cases of both areas,CD45RO,CD3,TIA-1 and CD56 negative in all the control group（20 cases）.CD20 positive in all the 20 ficial B-cell lymphomas cases and EBER was positive in 2 cases（10%）.Conclusion Higher level of expression appears strongly in Xining than that in Xi′an on cytotoxic proteins in nosal NK/T cell lymphomas in EBV infection,which indicate that EBV infection may have close relationship with nasal NK/T cell lymphomas in Xining.TIA-1 has a good sensitivity and specificity on the marker of NK/T cell lymphomas,but no significant difference with the geographical area.

关 键 词：NK/T 细胞淋巴瘤 免疫表型 TIA-1 EBER 

分 类 号：R739.62[医药卫生—肿瘤]