HSV-2型2.2kb潜伏相关转录子对Hela细胞生长特性的影响  

Effect of HSV-2 2.2 kb latency associated transcript on the growth characteristics of Hela cells

作　　者：毕建军[1] 杨慧兰[1] 樊建勇[1] 王颖[1] 李翠华[1] 梁洁[1] 

机构地区：[1]广州军区广州总医院皮肤科,广东广州510010

出　　处：《微生物学通报》2012年第9期1299-1305,共7页Microbiology China

基　　金：国家自然科学基金项目(No.30972666);全军医学科学技术研究"十一五"计划资助项目(No.06J008)

摘　　要：【目的】研究人单纯疱疹病毒2型(Herpes simple virus type 2,HSV-2)2.2 kb潜伏相关转录体(Latency associated transcript,LAT)对体外培养Hela细胞生长特性的影响。【方法】将真核表达质粒pcDNA3.1-2.2 kb LAT或pcDNA3.1转染Hela细胞后,观察细胞的生长情况。在4°C和43.5°C应激下,MTT法检测细胞活性,Hoechst 33258染色检测细胞凋亡。【结果】pcDNA3.1-2.2 kb LAT质粒转导入Hela细胞后,检测到2.2 kb LAT转录;细胞培养2 d转染pcDNA3.1-2.2 kb LAT细胞明显多于转染pcDNA3.1细胞数,两者细胞数分别为1.7±0.09×105/mL和1.45±0.14×105/mL,P<0.05。4°C下pcDNA3.1-2.2 kb LAT组和pcDNA3.1组细胞凋亡率分别为27.6%±5.7%和7.8%±1.5%,P<0.01;43.5°C下pcDNA3.1-2.2 kb LAT组和pcDNA3.1组细胞凋亡率分别为30.5%±4.6%和5.3%±2.0%,P<0.01。【结论】2.2 kb LAT具有促进细胞增殖,提高细胞活力和抗凋亡作用,提示它可能在HSV-2感染中发挥重要作用。[Objective] In this study,we investigated the influence of HSV-2 2.2 kb latency associated transcript on the growth characteristics of Hela cells in vitro.[Methods] We observed the growth of Hela cells transfected with recombinant expression plasmids pcDNA3.1-2.2 kb LAT along with pcDNA3.1 control.In 4 °C or 43.5 °C,vitality of cells and apoptosis rate of cells was detected by MTT and Hoechst33258 staining.[Results] The transcription of 2.2 kb LAT was detected in Hela cells transfected with pcDNA3.1-2.2 kb LAT.The number of cells transfected with pcDNA3.1-2.2 kb LAT was higher than that transfected with pcDNA3.1（1.7±0.09×105/mL and 1.45±0.14×105/mL,respectively,P〈0.05） at 2 days.The apoptosis rate of cells transfected with pcDNA3.1-2.2 kb LAT was lower than that transfected with pcDNA3.1（27.6%±5.7%和7.8%±1.5%,respectively,P〈0.01） at 4 °C.The apoptosis rate of cells transfected with pcDNA3.1-2.2 kb LAT was lower than that transfected with pcDNA3.1（30.5%±4.6%和5.3%±2.0%,respectively,P〈0.01） at 43.5 °C.[Conclusion] The Hela cells transfected with pcDNA3.1-2.2 kb LAT showed promoted proliferation,heightened vitality and reduced apoptosis.These experiments demonstrated that 2.2 kb LAT could play an important role in HSV-2 infection.

关 键 词：单纯疱疹病毒2型 生殖器疱疹 潜伏相关转录子 生长特性 凋亡 

分 类 号：R363[医药卫生—病理学]