MiR-200a在上皮性卵巢癌中的表达及临床意义  

Expression of microRNA-200a and its clinical significance in ovarian epithelial cancer

作　　者：徐韶华[1] 程文俊[2] 许培箴[1] 

机构地区：[1]南京医科大学附属常州市妇幼保健院妇瘤科,江苏常州213003 [2]南京医科大学第一附属医院妇科,江苏南京210029

出　　处：《实用临床医药杂志》2012年第15期47-49,53,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省常州市卫生局重大项目(ZD201113);南京医科大学科技发展基金重点项目(2011NJMU2311);江苏省科技厅自然科学基金面上项目(BK2010576);江苏省六大人才高峰基金(303070774IB09)

摘　　要：目的探讨miR-200a上皮性卵巢癌组织中的表达及与临床病理特征关系。方法采用Taqman茎环探针实时荧光定量PCR方法检测40例上皮性卵巢癌和30例良性上皮性卵巢肿瘤组织标本中miR-200a的表达,分析miR-200a的表达量与临床病理特征关系。结果 miR-200a在卵巢癌组织中的相对表达量显著高于良性卵巢肿瘤组织(P<0.01),在晚期卵巢癌组织中表达低于早期卵巢癌组织(P<0.05),在低分化卵巢癌中表达有下降趋势。miR-200a表达与病理类型及淋巴结转移无相关性。结论 miR-200a在上皮性卵巢癌组织中表达升高,在晚期卵巢癌中表达下降,提示其可能在卵巢癌的发生发展中起原癌基因的作用,并参与晚期卵巢癌的侵袭转移。Objective To investigate the expression and clinical significance of microRNA-200a in epithelial ovarian cancer.Methods The expressions of miR-200a in 40 epithelial ovarian cancer and 30 benign ovarian epithelial tumors were examined by stem-loop real-time RT-PCR.The correlations between the expression of miR-200a and clinicopathologic features（FIGO stage,pathological type,histological grading and lymphatic metastasis） were further analyzed.Results The expression of miR-200a was significantly higher in epithelial ovarian cancer than in benign ovarian epithelial tumors,but it was lower in advanced ovarian cancer than at early stage.Conclusion miR-200a may play a role as an oncogene in epithelial ovarian cancer,and is possibly correlated with the progression and metastasis at advanced stage.

关 键 词：MICRORNA miR-200a 上皮性卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]