痰热清注射液固形物中焦谷氨酸组分的RP-HPLC法测定  

Determination of P-Glu Content in Solid Substance of Tanreqing Injection by RP-HPLC

作　　者：杨清林[1] 傅亚[1] 

机构地区：[1]重庆科技学院化学化工学院,重庆401331

出　　处：《重庆师范大学学报（自然科学版）》2012年第5期67-70,共4页Journal of Chongqing Normal University:Natural Science

摘　　要：对痰热清注射液固形物中焦谷氨酸(P-Glu)的组分含量进行分析,通过对比P-Glu标准物质,采用反相高效液相色谱法(RP-HPLC)对不同批次的痰热清注射液的固形物进行测定,确定不同批次痰热清注射液中P-Glu含量。研究中所用色谱柱为Sionchrom ODS-BP(200 mm×4.6 mm,5μm),流动相为乙腈和3.5 mmol/L磷酸二氢钠缓冲液(两者体积比为1∶99),流速为1.0 mL/min,检测波长205 nm,柱温27℃。结果发现,不同批次痰热清注射液中P-Glu含量范围为0.5%～1.2%,平均含量近0.86%,故不同批次药品中P-Glu含量有一定差异。该方法能快速、有效地分析测定痰热清注射液固形物中P-Glu含量,对该注射液的质量控制有着重要意义。The content of P-Glu in solid substance in Tanreqing injection dose was analyzed. The content of P-Glu in solid sub- stance in Tanreqing by RP-HPLC was determined, and defined the content of P-Glu in the solid substance of different batches Tanreqing by comparing with reference of P-Glu. In this study the Chromatographic conditions was Sionchrom ODS-BP（200 mm X 4.6 ram, 5 tzm） column, acetonitrile-- 3.5 mmol/L phosphate buffer（volume ratio 1 ~ 99） with pH 3 as mobile phase, the flow rate was 1.0 mL/min, detection wavelength was set at 205 nm, the temperature of column was set at 27 C. The results showed that the Content of P-Glu in different batches were determined in the range of 0.5 %- 1.2 %, the average value was 0.86 %, it was indicated that the content of P-Glu in Tanreqing injection dose differs slightly in different batches. The method established in the paper was effective and stable, which is quite important to the quality control of Tanyeqing injection dose.

关 键 词：痰热清注射液 固形物 焦谷氨酸 反相高效液相色谱 

分 类 号：Q503[生物学—生物化学] R927.2[医药卫生—药学]