硫酸镁对6-羟基多巴胺诱导的帕金森病模型大鼠血中褪黑素的影响  

Effect of magnesium on serum melatonin in a rat model of Parkinson＇ s Disease

作　　者：马杰[1] 周全[1] 袁苏涛[2] 孟涛[1] 林玲[1] 

机构地区：[1]福建医科大学基础医学院生物化学与分子生物学系,福州350004 [2]:福建医科大学省立临床学院福建省立医院神经外科

出　　处：《中华实验外科杂志》2012年第9期1703-1705,共3页Chinese Journal of Experimental Surgery

基　　金：福建省自然科学基金资助项目（2010J01175）;福建省大学生创新性实验计划项目

摘　　要：目的观察晚期帕金森病（PD）大鼠血中褪黑素的变化，以及硫酸镁对这种变化的影响。方法6-羟基多巴胺（6-OHDA）损毁黑质纹状体通路制备PD模型，大鼠分为PD模型组（PD／H2O）、PD加镁组（PD／Mg）、对照组（对照组／H2O）、对照加镁组（对照组／Mg）。大鼠通过饮水摄入硫酸镁（每天0．36g/kg），4周后观察其对阿扑吗啡诱发的旋转行为，并采用酶联免疫吸附试验（ELISA）检测褪黑素水平。结果PD鼠血清褪黑素水平为（153．4±29．8）pg/L，明显高于对照组（77．2±13．7）pg/L；补镁后PD组和对照组大鼠的褪黑素水平分别降至（126．8±15．9）pgCL和（53．4±18．1）pg／L。PD／Mg组大鼠的旋转行为较PD／H2O组有改善。结论硫酸镁可缓解晚期PD鼠的运动症状，对PD鼠褪黑素的代偿性升高有抑制作用。Objective To explore the relationship between magnesium （Mg） and melatonin （MLT） by observation serum MLT levels in advanced Parkinson＇ s disease （PD） rats, and to clarify the effect of magnesium on serum melatonin in PD model. Methods PD rat model was established by a uni- lateral injection of 6-OHDA into the right substantia nigra pars compacta （SNc） and the right medial lore- brain bundle （MFB）. Rats of control group received saline injection. Twenty animals were divided into four groups. PD model （PD/H20） and control/H2 O groups were vehicle-treated rats, and received drinking wa- ter （magnesium sulphate vehicle） daily. Magnesium sulphate （MgSQ 0. 36 g/kg/day dissolved in drink- ing water） was administered in rats of PD/Mg and control/Mg groups for four weeks. Then apomorphine-in- duced rotational behaviour, serum MLT levels and histological changes were tested. Results The level of MLT in PD groups was （ 153.4 ±29. 8 ） pg/L, which distinctly higher than those in the controls （77.2 ± 13.7） pg/L, while the levels of MLT in PD model and control rats receiving Mg were decreased to （ 126. 8± 15.9） pg/L and （53.4 ± 18. 1 ） pg/L respectively, MLT levels appeared to correlate well with the frequency of apomorphine-induced rotations. The fi＇equency of rotations in PD/Mg group decreased com- pared with those in PD/H20 group. Conclusion The rotational behaviour in advanced PD rat might ameliorated by magnesium, and magnesium may partly inhibit compensatory increased MLT.

关 键 词：帕金森病 褪黑素 硫酸镁 

分 类 号：R742.5[医药卫生—神经病学与精神病学]