富含亮氨酸重复序列和免疫球蛋白样结构域基因3在肝癌细胞株中的表达及对细胞周期、侵袭性和凋亡的影响  

Expression of leucine-rich repeats and immunoglobin-like domains protein 3 gene in hepatocellular carcinoma cell lines and its influence on the cell cycle, apoptosis, and invasion

作　　者：齐永[1] 庄乾元[1] 彭鄂军[1] 李有元[1] 刘飞[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《中华实验外科杂志》2012年第9期1721-1723,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察富含亮氨酸重复序列和免疫球蛋白样结构域基L大J3（LRIG3）在3种肝癌细胞株HepG-2、LM3和7721中的表达，探讨LRIG3基因对肝癌细胞株HepG-2、LM3和7721的细胞周期、侵袭能力和凋产的影响。方法将过表达的LRIG3质粒和空白质粒经脂质体法分别转染肝癌细胞株HepG-2、LM3和7721中，相对廊分为实验组和对照组，采用逆转录-聚合酶链反应（RT—PCR）和Westernblot法检测LRIG3表达水平变化，采用流式细胞仪、Transwell法及原佗末端转移酶标记（TUNEL）等方法对细胞株的生物学行为进干亍分析。Objective To explore the eftect of overexpression of leucine-rich repeats and immuno- globin-like domains protein 3 （LRIG3） gene on the cycle, invasion and apoptosis of hepatocellular carcino- ma cell lines HepG-2, LM3 and 7721. Methods The plasmids of over-expressed LRIG3 and the blank plasmid serving as control were transduced into HepG-2, LM3 and 7721 cells. The expression levels of LRIG3 mRNA and protein were detected by using reverse transcription-polymerase chain reaction （RT- PCR） and Western blotting, and the changes in the cell cycle were determined by flow cytometry. The in- vasive capability was measured by Transwel] assay, and terminal-deoxynueleotidyl transferase mediated nick end labeling （TUNEL） method was used to measure apoptosis. Results As compared with the control group, the mRNA levels of LRIG3 were increased by 78.9% , 67.8% and 59.6% respectively, and their protein levels were increased by 91.2% , 79.90/o and 65.7% in HepG-2, LM3 and 7721 cells of experi- ment group, respectively （P〈0.05）. The average number of HepG-2, LM3 and 7721 cells over-express- ing LRIG3 passing through the inserted filter was decreased as compared with control group （ P 〈 0. 05 ）. The number of cells in S ＋ G2/M phase in HepG-2 and LM3 ceils of experiment group was significantly re- duced as compared with control group （ P 〈 0. 05 ）. The apoptosis rate of HepG-2, LM3 and 7721 cells was 35.7% , 39.5% and 30. 5% respectively in experiment groups, and that was 23.4% , 21.6% and 20. 2% respectively in control groups. There was significant difference between experiment group and control group （ P 〈 0. 05 ）. Conclusion The over-expression of LRIG3 gene could arrest the cell in S and G2/M phases, reduce the invasive capability of HCC, and accelerate apoptosis of HCC.

关 键 词：肝癌细胞株 免疫球蛋白 细胞周期 重复序列 结构域 亮氨酸 WESTERNBLOT法 逆转录-聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]