吲哚胺2，3-双加氧酶转染SMMC-7721细胞对肝癌血管形成的影响  

Effect of transfection of SMMC-7721 cells with indoleamine 2,3-dioxygenase on tumor angiogenesis of hepatocellular carcinoma cells

作　　者：何冬梅[1] 孙建莉[1] 张敏[1] 任晓静[1] 申慧琴[1] 武希润[1] 王琦[1] 

机构地区：[1]山西医科大学第二医院消化科,太原030001

出　　处：《中华实验外科杂志》2012年第9期1724-1726,共3页Chinese Journal of Experimental Surgery

基　　金：山西省科技攻关计划资助项目

摘　　要：肝细胞癌（HCC，简称肝癌）是最常见的恶性肿瘤之一，其进展快、恶性程度高、预后差、易复发转移。吲哚胺2，3-双加氧酶（IDO）是肝脏以外唯一可以催化L-色氨酸分子吲哚环裂解，使其沿犬尿酸途径代谢的限速酶。近年研究结果证实高表达IDO的肝癌转移与复发率均高于IDO低表达或不表达患者旧。。我们通过将IDO转染到SMMC-7721细胞与人脐静脉内皮细胞（HUVEC）共培养，观察血管形成结果。Objective To investigate the effect of indoleamine 2,3-dioxygenase (IDO) on tumor angiogenesis of hepatocellular carcinoma (HCC) cells and the possible mechanism.Methods By using the lipofectamineTM 2000 the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 were transfected into SMMC-7721 cells.After selection with G418,the single cell clone was chosen and cultured.The expression of IDO was detected by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting.Cells were divided into the non-transfection group,the empty plasmid transfection group,and the IDO transfection group.They were then co-cultured with human umbilical vein endothelial cells (HUVECs) by Transwell.Angiogenesis of HUVECs were observed,and the expression ofvascular endothelial growth factor (VEGF) in every group was detected.The cells in IDO transfection group were co-cultured with HUVECs,1-methyl-D-tryptophan (1-D-MT) (2.5 mmol/L) and tryptophan (200 mg/L) were added separately,and the angiogenesis of HUVECs and the expression of VEGF were observed.Results ( 1 ) The number of neovessels in the IDO transfection group was significantly more than that in the non-transfection group and the empty plasmid transfection group (24.56 ± 0.16,10.38 ± O.67,11.02 ± 0.14 ) ( P ＜ 0.05 ) ; The expression of VEGF in the IDO transfection group was significantly higher than in the non-transfection group and the empty plasmid transfection group (727.65 ±0.48),(512.05 ±1.11 ),(519.35 ±0.38) ng/L (P ＜0.05) ; (2) The number of neovessels in the IDO transfection group was more than that in the tryptophan group and the 1-D-MT group (24.84 ±0.54,15.14 ±0.24,14.68 ±O.84) P ＜ 0.05 ; The expression of VEGF in the IDO transfection group was higher than that in the tryptophan group and the 1 -D-MT group (727.65 ± 0.83 ),( 576.00 ± 5.83 ),(574.65 ± 0.79 ) ng/L ( P ＜0.05).Conclusion Stable transfection of pcDNA3.1-IDO plasmid into SMMC-7721 cells can influence the tumor angiogenesis by regulating the expression o

关 键 词：SMMC-7721细胞 3-双加氧酶 肝癌转移 血管形成 吲哚胺 转染 人脐静脉内皮细胞 L-色氨酸 

分 类 号：R735.7[医药卫生—肿瘤]