转染增殖细胞核抗原表位肽融合白细胞介素-18基因的树突状细胞刺激的T细胞对结肠癌细胞凋亡的影响  

Effect of T lymphocytes induced by dendritic cells modified by proliferating cell nuclear antigen- Epitope andinterleukin-18 gene on colonic cancer cells

作　　者：王磊[1] 王辉[1] 宋军[1] 李慧忠 李连涛 郑骏年 徐为[1] 

机构地区：[1]徐州医学院附属医院胃肠外科,江苏221002 [2]江苏省肿瘤生物治疗重点实验室

出　　处：《中华实验外科杂志》2012年第9期1756-1758,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察增殖细胞核抗原（PCNA）表佗肽融合白细胞介素18（IL-18）基㈥的树突状细胞（1）Cs）刺激的T淋巴细胞对结肠癌细胞凋亡的影响。方法实验分为DCs组、空载体组、PCNA表佗肽组、PCNA表化肽融合IL-18基因组。将空质粒（plRES—EGFP）、PCNA表位肽质粒[pIRES—EGFP-PCNA（6）]和PCNA表位肽融合IL-18基因质粒[pIRES—EGFP—PCNA（6）／IL-18]分别转染DCs。Objective To investigate the apoptosis of colonic cancer cells co-cultured with T lym- phocytes induced by dendritic cells modified by proliferating cell nuclear antigen （PCNA）-Epitope and inter- leukin-18 （IL-18） genes. Methods The experiment was divided into four groups, including DCs group, empty vector group, PCNA-Epitope transfection group, and PCNA-Epitope plus IL-18 gene group. Every group was operated according to DCs vs. T ratio （1： 10）. The empty vector plasmid （plRES-EGFP）, PCNA-Epitope plasmid [ plRES-EGFP-PCNA（6） ] and plasmid loaded with PCNA-Epitope and IL-18 gene plRES-EGFP-PCNA（6）/IL-18 ] were transfeeted into peripheral blood-derived dendritic cells by non-lip- id polymer-based reagent, then co-euhured with T lymphoeytes, respectively. The supernatants of immuno- cytes were cultured with colonic cancer cells according to the ratio of T lymphoeytes vs. colonic cancer cells （5：1, 10：1, 20：1, 40：1 ）. The apoptosis of colonic cancer cells was examined by using flow eytometry. Results The apoptosis of colonic cancer （：ells couht be induced in every group. The apoptosis in the plRES-EGFP-PCNA （6）/IL-18 group was significantly higher than in DCs group, empty vector group, and PCNA-Epitope transtetion group. The optimal ratio of T lymphoeytes vs. colonic cancer （;ells was 40： 1. Conclusion The supernatants of T lymphocytes induced by DCs that were treated with the PCNA-Epitope and 1L-18 gene can induce apoptosis of more colonic cancer cells.

关 键 词：结肠癌 树突状细胞 增殖细胞核抗原 白细胞介素-18 脱噬作用 

分 类 号：R735.35[医药卫生—肿瘤]