丙戊酸钠对胰腺癌细胞MICA／B表达的影响  

Valproate increases the expression of MICA/B in pancreatic cancer cells

作　　者：周峰[1] 石朋飞[1] 殷涛[1] 王春友[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《中华实验外科杂志》2012年第9期1775-1776,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30801100）;高等学校博士学科点专项科研基金资助项目（200804871112）

摘　　要：目的观察丙戊酸钠（VPA）对胰腺癌细胞NKG2D配体MICA和MICB表达的影响。方法1mmol／L的VPA孵育胰腺癌细胞24h，流式细胞术及实时定量聚合酶链反应（Real-timePCR）检测VPA处理前后胰腺癌细胞株PC-2及BxPC-3细胞表面NKG2D配体MICA和MICB的表达。结果胰腺癌细胞株PC-2及BxPC-3细胞表面MICA／B的阳性率分别为1．2％～1．5％、37．4％～42．5％；VPA处理后分别为3．2％～4．1％、62．4％～77．2％。PC-2及BxPC-3细胞MICAmRNA的表达水平为1．183±0．219、3．790±0．519，MICBmRNA的表达水平为1．020±0．025、3．267±0．183；经VPA处理后分别为3．173±0．456、16．700±0．534和2．543±0．385、14．730±0．370。结论VPA可以有效上调胰腺癌细胞株PC-2及BxPC-3细胞NKG2D配体MICA／B蛋白及mRNA的表达。Objective To study the effect of sodium valproate （VPA） on the expression of NKG2D ligands MICA and MICB in pancreatic cancer cells. Methods Pancreatic cancer cells were incu- bated with 1 mmol/L of VPA for 24 h, then, flow cytometry and real-time quantitative polymcrase chain reaction （Real-time PCR） were used to detect the expression of MICA/B in PC-2 and BxPC-3 cells of VPA-treated group and control group. Results The positive rate of MICAJB in PC-2 and BxPC-3 cells was 1, 2%-1.5% and 37.4%-42.5% ; and 3.2%-4. 1% and 62.4%-77.2% after VPA treatment. The MICA mRNA expression level in PC-2 and BxPC-3 cells was 1. 183 ＋ 0. 219 and 3. 790 ＋ 0. 519, and expression level of MICB mRNA was 1. 020＋0. 025 and 3. 267 ＋0. 183. After VPA treatment, the MICA/B mRNA expression levels in PC-2 and BxPC-3 cells were 3. 173 ＋0. 456 and 16. 700 ＋0. 534, and 2. 543 ＋0. 385 and 14. 730 ＋0. 370 respectively. Conclusion VPA can effectively increase the expression of MICA/B in PC-2 and BxPC-3 cells.

关 键 词：胰腺癌 MICA MICB 丙戊酸钠 

分 类 号：R735.9[医药卫生—肿瘤]