Rap2B在肾癌中的表达及其意义  

The expression and significance of Rap2B in suprarenal epithelioma

作　　者：陈仁富[1] 渠德宝 底洁卉 李连涛 陈菲菲 刘俊杰[1] 温儒民[1] 李望[1] 郑骏年 

机构地区：[1]徐州医学院附属医院泌尿外科,221002 [2]江苏省肿瘤生物治疗重点实验室

出　　处：《中华实验外科杂志》2012年第9期1799-1801,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省高校自然基金资助项目（10KJB320024）

摘　　要：目的探讨Rap2B在肾透明细胞癌中的表达及其意义。方法Westernblot法检测。肾透明细胞癌Caki-1、786-O细胞及人胚肾细胞株HEK293、人肾小管细胞株HK2的Rap2B表达。收集30例手术切除的肾癌组织与癌旁组织标本，Westernblot法、免疫组织化学法检测Rap2B表达。免疫组织化学检测75例肾透明细胞癌组织芯片的Rap2B表达，并分析其与病理分期的相关性。结果肾透明细胞癌细胞株的Rap2B蛋白表达显著高于正常肾小管细胞株（P〈0．05）；肾癌组织Rap2B表达水平明显高于癌旁组织（P〈0．05）；免疫组织化学显示，Rap2B主要定位于癌细胞胞膜上，正常细胞中很少。肾癌组织和芯片中Rap2B表达均显著高于癌旁组织（P〈0．05），组织芯片显示Rap2B表达与病理分期无相关性。结论肾透明细胞癌Rap2B表达水平增高。Objective To investigate the expression and significance of Rap2B in human suprare- nal epithelioma. Methods The expression of Rap2B protein in human suprarenal epithelioma cells was detected by Western blotting. Suprarenal epithelioma and para-cancer of renal tissues were obtained and ex- pression of Rap2B protein was examined by Western blotting. The expression of Rap2B in suprareual epithe- lioma tissues and surrounding normal tissues were examined by immunohistochemical technique, whether there was significant correlation of the Rap2B protein expression levels with clinical degree and patient a- ges. Results The expression of Rap2B protein was higher in the suprarenal epithelioma cell and suprarenal epithelioma tissues than that in normal renal cell and in surrounding normal tissues by Western blotting, sig- nificantly. The result of immunohistechermial analysis indicated the expression level of Rap2B were higher in suprarenal epithelioma tissues than in normal tissues significantiy. The study confirmed the predominant localization of Rap2B in plasma membrane of the carcinoma cell, and only little part was localization in plasma membrane of the para-caneer renal tissues. Conclusion Our data demonstrated that the expressions of Rap2B in suprarenal epithelioma cells and tissues were higher than normal cells and tissues.

关 键 词：Rap2B 肾透明细胞癌 

分 类 号：R737.11[医药卫生—肿瘤]