医用臭氧对非机械压迫性坐骨神经痛模型中异常自身免疫反应的影响  

Experimental study on abnormal autoimmunity in sciatica model

作　　者：刘成[1] 寿康全[1] 付纳新[1] 李坚[1] 黄晖[1] 滕宇[2] 

机构地区：[1]三峡大学第三临床医学院葛洲坝中心医院骨科,宜昌443002 [2]华中科技大学同济医学院附属协和医院骨科

出　　处：《中华实验外科杂志》2012年第9期1823-1825,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30973027,81171684）

摘　　要：目的观察医用臭氧对非机械压迫性坐骨神经痛模型中异常自身免疫反应的影响。方法在SD大鼠身上建立非机械压迫性坐骨神经痛模型，采用臭氧局部注射干预该模型，以医用纯氧作阴性对照，并在干预前后分别运用透射比浊法检测大鼠血清中免疫球蛋白IgG、IgM的含量，酶联免疫吸附试验（ELISA）法测定血清中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6、IL-12的含量；采用免疫组织化学染色观察移植髓核中抗原抗体复合物沉积；运用二喹啉甲酸（BCA）蛋白定量法观察坐骨神经中磷脂酶A2（PLA2）的活性。结果经过臭氧注射干预后，大鼠模型后肢机械缩爪阈值明显升高[（88．7±4．4）mmHg（1mmHg=0．133kPa），P〈0．01]，血清中IgG、IgM水平[（4．11±0．12）、（0．35±0．11）g／L]以及炎性细胞因子TNF-α、IL-6、IL-12的含量[（37．25±4．61）、（101．60±49．60）、（42．11±23．51）pg／L]均出现差异有统计学意义的下降（P〈0．05），移植髓核中抗原抗体复合物阳性沉积表达率有明显降低（10％，P〈0．01），其相应节段坐骨神经中炎性始动因子PLA2活性显著下降[（0．0143±0．0027）μmoL／（min·L）]。结论医用臭氧能够在一定程度上抑制坐骨神经痛模型中异常的自身免疫反应。Objective To investigate the influence of medical ozone on the abnormal autoimmunity of sciatica model on the animal. Methods An non-mechanical pressure sciatica model was established on Sprague Dawley rats. Then medical ozone was used for treatments to the animal, and medical purified oxy- gen was used as the negative control. The mechanical withdrawal thresholds of posterior limbs were meas- ured. The immunoglobulin G and immunoglobulin M in serum were examined by using urbidimetry. The presence and distribution of antigen-antibody complexes in nucleus pulposus were examined by using immu- nohistochemistry. The levels of tumor necrosis factor （TNF）-c~, interleukin （IL）-6 and IL-12 were deter- mined by using enzyme linked immunosorbent assay, and we also used the BCA method to analyze the ac- tivity of PLA2 in the sciatic nerve. Results As compared with the model control group, the medical ozone group had significantly higher posterior limb＇ s retracting thresholds [ （ 88.7 ± 4.4） mm Hg （ 1 mm Hg = 0. 133 kPa） ,P 〈0. 01 ] , weaker immune inflammatory reaction of the nerve root, lower levels of the immu- noglobulin G and M [ （4. 11 ± 0. 12）, （0. 35 ± 0. 11 ） g/L,P 〈 0. 051 and TNF-α, IL-6, IL-12 in the serum [ （37. 25 ±4. 61 ）, （ 101.60 ±49. 60）, （42. 11 ±23.51 ） pg/Ll, lower expression of antigen-antibody com- plexes in the autologous nucleus pulposus （10% ,P 〈0. 01 ）, and lower activity of PLA2 in the sciatic nerve [ （0. 0143 ±0. 0027） μmoL/（ rain. L）]. Conclusion Medical ozone injection could inhibit the abnormal autoimmunity of sciatica model to an extent.

关 键 词：坐骨神经痛 免疫 医用臭氧 

分 类 号：R741[医药卫生—神经病学与精神病学]