非小细胞肺癌经典人类白细胞抗原I下调与淋巴结转移的关系及其调控机制  

The mechanism and relationship between down-regulated classical human leukocyte antigen class I expression and metastatic lymph node lesions in non-small cell lung cancer

作　　者：宋福杰[1] 刘相燕[1] 王洲 

机构地区：[1]山东大学附属省立医院胸外科,济南250021

出　　处：《中华实验外科杂志》2012年第9期1837-1839,共3页Chinese Journal of Experimental Surgery

基　　金：山东省自然科学基金资助项目（Y2007C106）

摘　　要：目的探讨非小细胞肺癌（NSCLC）经典人类白细胞抗原I（HLA—I）类抗原表达下调与淋巴结癌转移的相关性及其调控机制。方法应用流式细胞术检On,065例NSCLC原发灶及31例淋巴结转移灶经典HLA—I类抗原的表达，应用逆转录．聚合酶链反应（RT—PCR）技术检测各3l例非小细胞肺癌原发灶、淋巴结转移灶抗原处理相关转运体1（TAPl）mRNA、潜伏膜蛋白2（LMP2）mRNA的表达，根据不同的变量类型及目的，采用不同的统计方法分析。结果淋巴结转移灶经典HLA—I类抗原的表达率（15．35±6．24）％明显低于肺癌原发灶（39．68±12．46）％（t=2．06，P〈0．05），且与肺癌原发灶经典HLA—I类抗原表达下调明显呈正相关（rs=0．487，P〈0．01）。经典HLA—I类抗原表达下调与阳性表达的肺癌原发灶TAPlmRNA、LMP2mRNA表达率差异有统计学意义（P〈0．05）。经典HLA—I类抗原表达下调的淋巴结癌转移灶中TAPlmRNA、LMP2mRNA表达率明显低于肺癌原发灶（，=5．01及5．39，P〈0．05）。结论经典HLA—I类抗原表达下调是肺癌淋巴结转移的机制之一。TAPl、LMP2mRNA的表达缺陷是NSCLC原发灶及淋巴结转移灶经典HLA-I抗原表达下调的机制之一，在淋巴结癌转移灶表现更明显。Objective To detect the expression of classical human ledkocyte antigen （HLA） class I antigens in primary lesions and metastatic lymph nodes in non-small cell lung cancer （NSCLC） by using flow cytometry （FCM） , elucidate the correlation of classical HLA lass I antigen expression With primary lesions and metastatic lesions, and probe the relationship between loss of transporter associated with antigen processing 1 （TAP1）, latent membrane protein 2 （LMP2） mRNA and loss or down-regulated classical HLA class I antigen expression in tumor cells. Method＇s The expression of classical HLA class I anti- gens in 65 primary lesions in NSCLC and 31 metastatic cancer lesions in lymph nodes was examined by FCM. The expression of TAP1 and LMP2 mRNA in 31 primary lesions in NSCLC with classical HLA- I an- tigens downregulation or loss and 31 metastatic lesions in lymph nodes with down-regulation or loss of HLA- I antigens was detected by using reverse transcription-polymerase chain reaction （RT-PCR）. Results The expression rate of classical HLA class I antigens in 31 lymph node metastatic lesions [ （15.35 ± 6. 24）% ] was remarkably lower than that in primary lesions [ （39. 68 ± 12. 46）% ] （t = 2. 06, P 〈 O. 05 ） , and positive correlation existed between down-regulated classical HLA class I antigens in primary lesions in NSCLC with metastatic lesions in lymph nodes （r, = 0. 487 ,P 〈 0. 01 ）. The expression of TAP1 and LMP 2 mRNA was detected in 13 cases （41.9%） and 12 cases （38.7%） in 31 primary lesions, and 5 cases （ 16. 1% ） and 4 cases （ 12. 9% ） in 31 metastatic lesions, respectively. There was significant difference in the expression of TAP1 and LMP 2 mRNA between primary lesions and metastatic lesions （ P 〈 O. 05 ）. Conclusion There is a positive correlation between the expression of classical HLA class I antigens in primary lesions in lung cancer and metastatic lesions in lymph nodes. Loss or down-regulation of classical HLA class I antigen expression

关 键 词：非小细胞肺癌 抗原表达 淋巴结 转移 抗原处理相关转运体1 MRNA 潜伏膜蛋白2 MRNA 

分 类 号：R734.2[医药卫生—肿瘤]