蝎毒多肽提取物对卵巢癌SKOV3细胞HPA与VEGF抑制作用的实验研究  

Inhibitive Effect of Polypeptide Extract from Scorpion Venom(PESV) on the Expression of HPA and VEGF in SKOV3 Cell Lines

作　　者：李晓辉[1] 张维东[1] 王兆朋[1] 张月英[1] 贾青[1] 王朝霞[1] 

机构地区：[1]山东省医学科学院基础医学研究所病理室,山东济南250062

出　　处：《现代生物医学进展》2012年第24期4624-4626,4657,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(81073102;30873408);山东省自然科学基金资助项目(ZR2010HQ003;2009ZRC03044);济南市科学技术发展计划(201004012)

摘　　要：目的:研究蝎毒多肽提取物(polypeptide extract from scorpion venom,PESV)对卵巢癌SKOV3细胞HPA与VEGF表达的影响,进一步探讨其抗血管生成的分子机制。方法:卵巢癌SKOV3细胞分为对照组、PESV组,免疫组织化学方法检测各组HPA、VEGF的表达,western-blot方法检测各组HPA在蛋白水平的表达。结果:与对照组相比,PESV组HPA、VEGF表达明显降低(P<0.05),进一步检测发现PESV组HPA在蛋白水平表达亦明显下调(P<0.05)。结论:PESV能够抑制卵巢癌SKOV3细胞的生长,其机制可能与抑制HPA、VEGF的表达有关。Objective： To investigate the effect ofpolypeptide extract from scorpion venom（PESV） on the expression of HPA and VEGF in SKOV3 cell lines, and to explore the antiangiogenic mechanisms. Methods： SKOV3 cells were divided into control group and PESV group. Immohistochemistry was performed to determine the expression of HPA and VEGF in each group.Western-blot was used to detect the protein expression of HPA. Results： Compared with that in the control group, the expression of HPA and VEGF was stgllitt- cantly downregulated in PESVgroup, and the amount of HPA on protein level reduced significantly. Conclusions： PESV can inhibit the growth of SKOV3 cells, and the mechanism is probably related with the inhibition of the expression of HPA and VEGF.

关 键 词：蝎毒多肽提取物 卵巢癌 HPA VEGF 

分 类 号：R737.31[医药卫生—肿瘤]