高效液相色谱法同时测定沙棘果中槲皮素、山柰酚和异鼠李素的含量  

Simultaneous determination of quercetin,kaempferol and isorhamnetin in Hippophae rhamnoides L.fruit by HPLC

作　　者：樊鑫梅[1] 申雪丽[1] 闫丽丽[1] 王新春[1,2] 尹俊涛[3] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]石河子大学医学院第一附属医院,新疆石河子832008 [3]石河子开发区神内食品有限公司,新疆石河子832000

出　　处：《中国医院药学杂志》2012年第17期1343-1346,共4页Chinese Journal of Hospital Pharmacy

基　　金：新疆建设兵团火炬计划(编号:2011BD021)

摘　　要：目的:采用高效液相色谱法同时测定沙棘果中槲皮素、山柰酚和异鼠李素的含量。方法:Kromasil-C18(250 mm×4.6mm,5μm)色谱柱,流动相为乙腈(A)-0.6%磷酸(B)水溶液,线性梯度洗脱(0～15 min,33%→32%A;15～30 min,32%→31%A),流速1.0mL.min-1,柱温30℃,检测波长370 nm。结果:槲皮素,山柰酚和异鼠李素的质量浓度分别在1.68～26.88,1.52～24.32和1.76～28.16μg.mL-1范围内线性关系良好,平均回收率分别为98.55%,97.63%和97.98%,RSD分别为1.48%,1.66%和1.54%。结论:所建立的方法简单易行,准确可靠,可用于沙棘果中槲皮素、山柰酚和异鼠李素的含量测定。OBJECTIVE To develop an HPLC method for simultaneous determination of three flavonoids such as quercetin,kaempferol and isorhamnetin in Hippophae rhamnoides L.fruit.METHODS The column was Kromasil-C18（250 mm×4.6 mm,5 μm）,the mobile phase was acetonitrile（A）-0.6% phosphoric acid solution（B） with linear gradient elution（0-15 min,33%→32% A;15-30 min,32%→31% A）,the flow rate was 1.0 mL·min-1,the column temperature was 30 ℃ and the detection wavelength was 370 nm.RESULTS The method had good linear relationship within the range of 1.68-26.88 μg·mL-1 for quercetin,1.52-24.32 μg·mL-1 for kaempferol,and 1.76-28.16 μg·mL-1 for isorhamnetin.The average recoveries of quercetin,kaempferol and isorhamnetin were 98.55%,97.63% and 97.98%,respectively and the RSD were 1.48%,1.66% and 1.54%,respectively.CONCLUSION This method is simple,accurate and reliable.It is can be used to determine the contents of quercetin,kaempferol and isorhamnetin in Hippophae rhamnoides L.fruit.

关 键 词：沙棘果 槲皮素 山柰酚 异鼠李素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]