肺癌细胞株中层粘连蛋白5多态性与其表达水平的关系  

Relationships between Polymorphisms of Laminin 5 and Expression Level in Lung Cancer Cell Lines

作　　者：安社娟[1] 陈志红[1] 朱建权[1,2] 严红虹[1] 杨素清[1] 陈世良[1] 孟薇[1] 苏健[1] 黄迎[1] 谢至[1] 郭伟浜[1] 吴一龙[1] 

机构地区：[1]广东省人民医院医学研究中心、广东省医学科学院、广东省肺癌研究所,广州510080 [2]天津医科大学附属肿瘤医院肺部肿瘤科,天津300060

出　　处：《循证医学》2012年第4期248-252,共5页The Journal of Evidence-Based Medicine

基　　金：国家自然科学基金资助项目(81101549);广东省自然科学基金资助项目(S2011010000792)

摘　　要：目的探讨肺癌细胞株中层粘连蛋白5(LN5)5′非翻译区多态性与其表达水平的关系。方法应用测序及实时定量PCR方法检测12株肺癌细胞株中LN55′非翻译区多态性和LN5基因mRNA表达水平,分析相互之间的关系。结果 LN55′非翻译区-183G/A中GA基因型的LN5mRNA表达水平明显高于GG基因型(t=7.136,P<0.001)。本研究中尚未发现LN5基因-6C/A和-89A/G多态性位点与其mRNA表达的相关性。结论本研究提示LN5启动子区-183G/A多态性位点具有转录调控功能,为进一步的机制探讨提供了依据。Objective To explore the relationships between the polymorphisms in the 5＇-UTR region of laminin 5 （LN5） and the expression level in lung cancer cell lines. Methods The polymorphisms of LN5 and the mRNA expression levels in 12 lung cancer cell tines were detected, using sequencing and real-time PCR methods, respectively, the relationships between them were analyzed. Results Cell lines with GA genotype of -183G/A polymorphisms have a higher mRNA expression level of LN5 than the cell lines with GG genotype （t= 7.136,P〈0.001 ）. No relationships of -6C/A and -89A/G polymorphisms and the mRNA expression levels were found in this study. Conclusions This study suggestes that -183G/A polymorphisms of LN5 gene maybe regulate the transcriptional level, and these results provide the basic evidence for the further mechanism study.

关 键 词：肺癌 层粘连蛋白5 多态性 

分 类 号：R734.2[医药卫生—肿瘤]