微波等离子体常压解吸电离质谱法快速检测化学药剂中的活性成分  

Rapid Detection of Active Pharmaceutical Ingredients in Drugs by Microwave Plasma Atmospheric Pressure Desorption Ionization Mass Spectrometry

作　　者：张体强[1,2] 金伟[1] 周建光[1] 张莹[1] 朱志强[3] 周炜[3] 金钦汉[1] 

机构地区：[1]浙江大学工业控制技术国家重点实验室,智能系统与控制研究所,分析仪器研究中心,杭州310058 [2]浙江大学化学系,杭州310027 [3]东华理工大学江西省质谱科学与仪器重点实验室,南昌330013

出　　处：《高等学校化学学报》2012年第9期1938-1944,共7页Chemical Journal of Chinese Universities

基　　金：国家重大科学仪器设备开发专项(批准号:2011YQ140150);浙江省重大科技项目(批准号:2008C01053-5)资助

摘　　要：构建了一种新型电离源——微波等离子体常压解吸电离源,等离子体由微波等离子体炬产生,工作气体为Ar气,微波频率为2450 MHz,该离子源可在大气压下产生稳定的等离子体.将该电离源与具有大气压接口的Corsair API-TOF型飞行时间质谱仪结合,实现了化学药剂中单一或多种主要活性成分的快速分析,在手动进样条件下,检测速度可达每小时360次.在微波等离子体环境下,活性物质成盐时母体化合物上结合的酸性物质可被直接除掉,谱图中主要离子为母体化合物的准分子离子[M+H]+,便于识别.微波等离子体常压解吸电离质谱法无需化学试剂,具有实时、快速及无污染等特点,为药剂研发及化学工业提供了一种新的检测技术.A new ionization source, termed microwave plasma atmospheric pressure desorption ionization （MWPAPDI） source, was developed. The stable plasma was generated by microwave plasma torch working at 2450 MHz at atmospheric pressure. Ar was used as working gas. The source was successfully coupled with a Corsair API-TOF time of flight mass spectrometer, and the active pharmaceutical ingredients in drugs were rapidly characterized. The speed was allowed as fast as approximate 360 samples per hour via manual sampling. In the microwave plasma environment, the hydrogen bound moiety of the active ingredients of the drug preparations was directly removed, as a result, the quasi-molecular ions of active component compounds [ M＋H] ＋ became the main ions and gave simple mass spectra. Without chemical reagents, MWPAPDI has the feature for direct analysis of samples with high throughput, no pollution and in real time. Therefore, the method is believed to be a useful technique for drug discovery and chemical industry.

关 键 词：微波等离子体常压解吸电离 质谱 药剂 活性成分 快速分析 

分 类 号：O657.63[理学—分析化学]