一株嗜蜡嗜胶质基因工程菌ZH_(20)的构建与评价  

Reconstruction of a genetic engineering bacteria ZH_(20) and its evaluation for paraffin and colloid biodegradation

作　　者：宋永亭[1,2] 王慧[2] 宋欣[2] 王静[2] 马挺[3] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266100 [2]中国石化股份胜利油田分公司采油工艺研究院,山东东营257000 [3]南开大学生命科学学院,天津300071

出　　处：《中国石油大学学报（自然科学版）》2012年第4期172-176,共5页Journal of China University of Petroleum(Edition of Natural Science)

基　　金：国家'863'重点项目(2009AA063500)

摘　　要：利用DNA转化法将嗜胶质菌的功能基因转入嗜蜡菌中,成功构建一株嗜蜡、嗜胶质基因工程菌ZH20,分析此菌株的生物特性及高温下对原油的作用。结果表明:ZH20的生长特性与受体菌基本相同,最高耐受温度为75℃,对pH值的适应范围较广(4～10),对盐(NaCl)的最大耐受量为12.5%;基因工程菌ZH20不仅保留受体菌的嗜蜡性能,同时还获得供体菌的嗜胶质性能,能够有效降低原油凝固点和黏度。A genetic engineering bacteria ZH2owas reconstructed by transforming genomic DNA from a colloid thirsty bacterium to a paraffin thirsty bacterium for enhancing biodegradation of paraffin and colloid. The biological characteristics of ZH20 and its effect on crude oil at high temperature were evaluated. The results show that the biological characteristics of ZH20 are analogous to the rec, ipient bacterium-the paraffin thirsty bacterium. ZH20 is stable at 75 ℃, acid or alkali stable at a pH range of 4-10, and NaCl stable at a concentration of 12.5%. The genetic engineering bacteria ZH20 inherits both paraffin thirsty and colloid thirsty characteristics, and it can simuhaneously and effectively reduce freezing point and viscosity of crude oil.

关 键 词：微生物采油 基因工程菌 生物降解 清防蜡 降黏 

分 类 号：X172[环境科学与工程—环境科学] TE357.9[石油与天然气工程—油气田开发工程]