抗阻、耐力运动对大鼠骨骼肌Perilipin、HSL基因表达及血清FFA含量的影响  

Effects of Resistance and Endurance Exercise on Perilpin and HSL in Gene Expression as Well as Blood Serum Among Rat's Skeletal Muscle

作　　者：张可斌[1] 单凤军[2] 

机构地区：[1]钦州学院体育学院,广西钦州535000 [2]周口师范学院体育系

出　　处：《现代预防医学》2012年第18期4792-4794,共3页Modern Preventive Medicine

摘　　要：目的研究长期不同强度运动对大鼠胫骨前肌HSL、Perilipin基因表达及血清FFA含量的影响。方法 46只雄性SD大鼠,适应练习时淘汰6只。将40只大鼠随即分为安静对照组(C组)、中等耐力运动组(M组)、大强度运动组(N组)、抗阻训练组(R组)。10周训练结束后采用生化方法测定各组大鼠血清FFA含量,RT-PCR法测定ATGL、HSL基因表达。结果与C组相比,运动各组血清中FFA含量增加(P﹤0.05,P﹤0.01);R组、N组HSL基因表达上调(P﹤0.05),其中R组基因表达量最多;R组、N组Perilipin基因表达下调(P﹤0.05),其中以R组基因表达最少。结论抗阻、耐力训练均可以导致骨骼肌HSL基因表达上调,Perilipin基因表达下调,血清FFA含量增加。抗阻、大强度耐力训练显著提高HSL基因表达水平,降低Perilipin基因表达水平,提示Perilipin基因表达的下调似乎可以促进HSL基因表达上调,提高骨骼肌脂肪水解能力。OBJECTIVE To study the effects of Iong-term training with different intensities on Perilpin and HSL in gene expression and blood serum in protein content among rat＇s skeletal muscle. METHODS 46 SD male rats were collected in adap- five training, and 6 rats were excluded. 40 rats were randomly divided into group C （sedentary control group）, group L （low- intensity movement）, group M （medium-intensity movement） and group N （high-intensity movement）. The methods of Bio- chemistry and Rel-Time PCR were carried out to test the expression of ATGL and HSL. RESULTS Compared with Group C, FFA in the blood serum in each group was significantly higher （P 〈 0.05, P 〈 0.01 ）. The HSL expression in the R and N groups significantly increased （P 〈 0.05）. The expression of R gene expressed most. The Perilpin expression in R and N groups de- creased （P 〈 0.05）, and expression of R gene express least. CONCLUSION The long-term training with different intensities can lead to the up-regulating of HSL in gene expression among rat＇s tibialis anterior and down-regulating of Perilipin in protein content. Medium intensity, especially the high-intensive long training, could dramatically improve the levels of Perilpin in gene expression. High-intensify training could enhance the HSL in gene expression and lower the content of peillipin, and enhance the fat hydrolysis abihty in skeletal muscle.

关 键 词：胫骨前肌 FFA HSL PERILIPIN 基因表达 蛋白含量 

分 类 号：R34[医药卫生—基础医学]