过氧化氢对Hela肿瘤细胞毒性和衰老的影响  

Influence of Peroxide on the cytotoxicity and senescence of Hela cells

作　　者：陈婉嫦[1] 何冬梅[2] 李江滨[1] 郑若洋[1] 余涌珠[1] 欧嘉文[1] 谭荧飞[1] 

机构地区：[1]广东医学院,广东东莞523808 [2]广东省惠州市中医医院,广东惠州516001

出　　处：《中国当代医药》2012年第25期9-10,共2页China Modern Medicine

基　　金：广东省湛江市科技攻关项目(2008C03015);高等学校特色专业建设点经费(TS10466)

摘　　要：目的体外实验探讨过氧化氢对Hela肿瘤细胞毒性和衰老的影响。方法不同浓度的过氧化氢处理Hela细胞,CCK-8法检测细胞毒性,β-半乳糖苷酶染色法测定细胞衰老,测定平均荧光强度(MFI)反映细胞产生活性氧的水平。结果细胞增殖实验显示,400μmol/L、800μmol/L、1600μmol/L对体外培养的Hela细胞有不同程度的细胞毒性,最高达89.0%。过氧化氢处理后,细胞衰老率和活性氧产生水平随过氧化氢浓度升高而增高,呈现量效关系。结论过氧化氢对Hela细胞有明显的细胞毒性,还能诱导其衰老和影响活性氧的产生水平。Objective To investigate the influence of Peroxide on the cytotoxicity and senescence of Hela cells in vitro. Methods Used the CCK-8 method to detect cytotoxicity and 13-galactosidase staining to determine the senescence of Hela Cells. The reactive oxygen species （ROS） produced in cell was detected by Mean fluorescence intensity（MFI）. Results The Peroxide at 400 μmol/L, 800 μmol/L, 1 600 μmol/L had different cytotoxicity, the highest was 89.0%, on Hela cells in vit- ro, the rate of cell aging and the ROS produced was increasing with the increasing concentration of Peroxide, present con- centration-respones relationship. Conclusion The Peroxide has cytotoxicity on Hela cells in vitro and it can also promotes the cell senescence and the ROS produced.

关 键 词：过氧化氢 HELA细胞 细胞增殖 细胞衰老 活性氧 

分 类 号：R-33[医药卫生]