A Novel 3-D Nano-assembly Bacteria Based Biosensor for Enhanced Detection of Heavy Metal Pollutants  

作　　者：Mina Souiri Ibtissem Gammoudi Laurence Mora Hafedh Ben Ouada Thierry Jouenne Nicole Jaffrezic-Renault Corinne Dejous Ali Othmane Anthony C. Duncan 

机构地区：[1]Biophysic Laboratory, LIMA, Faculty of Medicine of Monastir, University of Monastir, Monastir 5019, Tunisia [2]Department of Bioengineering of Cardiovascular Polymers, Galilee Institute, Villetaneuse 93430, France [3]Laboratory of the Interfaces of the Advanced Materials, Faculty of Sciences of Monastir, Monastir 5000, Tunisia [4]Proteomic Platform, Faculty of Sciences of Rouen, Mont Saint A ignan, Cedex 76821, France [5]Analytical Sciences Laboratory, Claude Bernard Lyon 1 University, Villeurbanne Cedex 69622, France [6]University of Bordeaux, Talence 33405, France

出　　处：《Journal of Environmental Science and Engineering(A)》2012年第7期924-935,共12页环境科学与工程（A）

摘　　要：Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficult but important task. The current major limitation lies in the poor detection limits of the targeted pollutant＇s trace concentrations by the available conventional techniques. In order to elaborate a novel ＂living＂ self assembled electrochemical 3-D biosensor, the authors propose a new concept to overcome this shortcoming. The advantages of the properties of polyelectrolyte-functionalized NBs （nanobeads） are combined along with the use of non covalently strongly bound micro-organisms. The designed 3-D biosensor is all the more promising as it has showed a significantly improved sensitivity. In fact, the detection limits of the tested heavy metals （cadmium and mercury） were as low as 1.0 × 10^-12 mol.L-1 and six to seven orders of magnitude lower than those provided by conventional 2-D biosensors. Furthermore, it is potentially applicable to a wide range of bioreceptor-pollutant detection systems.

关 键 词：BIOSENSOR nanobeads PEM heavy metals E. coli EIS （electrochemical impedance spectroscopy）. 

分 类 号：X132[环境科学与工程—环境科学] TP212.3[自动化与计算机技术—检测技术与自动化装置]