酶法辅助提取南板蓝叶中靛玉红的工艺研究  

Study on Enzyme-assisted Extraction of Indirubin from Baphicacanthus cusia(Nees) Bremek Leaves

作　　者：黄凤婷[1] 马永良[2] 林春颖[1] 熊清平[3] 

机构地区：[1]广州中医药大学第三附属医院芳村分院,广东广州510360 [2]中山大学孙逸仙纪念医院,广东广州510120 [3]淮阴工学院,江苏淮安223003

出　　处：《安徽农业科学》2012年第26期12866-12867,12879,共3页Journal of Anhui Agricultural Sciences

基　　金：广州市卫生局中医科研课题资助项目(2009-A-50);广东省中医药局建设中医药强省科研课题项目(2009303)

摘　　要：[目的]研究酶法提取关键参数对南板蓝叶中靛玉红提取工艺的影响。[方法]采用HPLC测定靛玉红的含量,并考察酶用量、酶解pH值和酶解时间对提取率的影响,并优选出了最佳工艺条件。[结果]南板蓝叶中靛玉红酶法辅助提取的最佳工艺条件为:酶用量25 U/g,酶解pH值4.8,酶解时间6 h;在此条件下,靛玉红的Z值为7.02%。[结论]酶法辅助提取法适用于南板蓝叶中靛玉红的提取。[ Objective ] To observe the key parameters of enzymatic extraction that impact extraction of indirubin from Baphicacanthus curia （Nees） Bremek leaves. [ Method] The content of indirubin was determined using HPLC. Effects of enzyme dosage, hydrolysis pH and hydrolysis time on extraction rate of indirubin were investigated with the aim of optimizing its extraction technology. I Resultl The optimal enzyme-assisted extraction conditions of indirubin from B. cusia leaves were the combination of enzyme dosage 25 U/g, hydrolysis pH 4.8 and hydrolysis time 6 h. Under the above conditions, the Z value of indirubin was 7.02%. [Conclusion] Enzyme-assisted extraction method is suitable for extraction of indirubin from B. cusia leaves.

关 键 词：酶法辅助提取 靛玉红(Indirubin) 南板蓝根(Baphicacanthus cusia(Nees) Bremek) 

分 类 号：S567[农业科学—中草药栽培]