量子点免疫荧光技术检测基质Cav-1、LDH-B和PKM2蛋白在人肺腺癌中的表达及其临床意义  

The expressions of stromal Cav-1, LDH-B and PKM2 proteins in human pulmonary adenocarcinoma tissues detected by quantum dots-based immunofluorescence technique and their clinical significance

作　　者：赵朋月[1] 宋建[2] 刁路明[1] 罗小青[2] 黄燕华 王则胜[4] 陈洪雷[1] 

机构地区：[1]武汉大学基础医学院病理学教研室,湖北武汉430071 [2]湖北医药学院附属襄阳市第一人民医院肿瘤科,湖北襄阳441000 [3]武汉市肿瘤纳米诊断工程技术研究中心分子病理科,湖北武汉430070 [4]武汉大学人民医院病理科,湖北武汉430060

出　　处：《肿瘤》2012年第9期731-737,共7页Tumor

基　　金：国家自然科学基金资助项目(编号:30900652)

摘　　要：目的:检测基质Cav-1(caveolin-1)、M2型丙酮酸激酶(pyruvate kinase M2,PKM2)和左旋乳酸脱氢酶B(L-lactate dehydrogenase B,LDH-B)在人肺腺癌(pulmonary adenocarcinoma,PAC)组织中的表达,探讨基质Cav-1对PAC侵袭和转移的影响。方法:利用量子点免疫荧光组织化学技术检测68例PAC组织和16例非癌变肺组织中基质Cav-1、LDH-B和PKM2的表达,同时应用量子点免疫荧光双标法检测基质Cav-1和α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的共表达。结果:基质Cav-1、LDH-B和PKM2在PAC组织中的阳性表达率分别为58.8%、54.4%和35.3%,在非癌变肺组织中的阳性表达率分别为100.0%、12.5%和6.3%,差异有统计学意义(P<0.05)。Cav-1、LDH-B和PKM2蛋白的表达与PAC患者的临床病理参数无相关性(P>0.05)。基质Cav-1与LDH-B和PKM2蛋白的表达呈负相关(P<0.05,r_s=-0.406;P<0.05,r_s=-0.320),基质LDH-B与PKM2蛋白的表达之间呈正相关(P<0.05,r_s=0.367)。结论:基质Cav-1表达缺失可能促进PAC的形成,其机制可能与上调LDH-B和PKM2的表达有关。Objective： To examime the expressions of caveolin-1 （Cav-1）, pyruvate kinase M2 （PKM2） and L-lactate dehydrogenase B （LDH-B） proteins in human pulmonary adenocarcinoma （PAC） tissues, and to investigate the effect of stromal Cav-1 expression on the invasion and metastasis of PAC. Methods： The expressions of stromal Cav-1, LDH-B and PKM2 proteins in PAC tissues from 68 cases and non-cancerous lung tissues from 16 cases were detected by quantum dots-based immunofluorescent histochemistry, and the co-expressions of stromal Cav-1 and α-smooth muscle actin （α-SMA） proteins were detected by quantum dots-based double-labeling immunofluorescence method. Results： The positive rates of stromal Cav-1, LDH-B and PKM2 expression were 58.8%, 54.4% and 35.3% in the PAC tissues, respectively, while which were 100%, 12.5% and 6.3% in the non-cancerous lung tissues, respectively. The difference between PAC and non-cancerous lung tissues was significant （P 〈 0.05）. There was no significant relationship between the expressions of stromal Cav-1, LDH-B and PKM2 proteins and the clinicopathological characteristics of patients with PAC （P 〉 0.05）. The expression of stromal Cav-1 was negatively correlated with the expressions of stromal LDH-B and PKM2 （P 〈 0.05, rs = –0.406; P 〈 0.05, rs = –0.320）, but the expression of stromal LDH-B was positively correlated with the expression of stromal PKM2 （P 〈 0.05, rs = 0.367）. Conclusion： The loss of stromal Cav-1 expression may promote PAC formation, and this mechanism may be associated with the up-regulation of LDH-B and PKM2 expressions.

关 键 词：肺肿瘤 能量代谢 量子点 癌相关成纤维细胞 CAVEOLIN-1 

分 类 号：R734.2[医药卫生—肿瘤]