腺苷酸活化的蛋白激酶通过SPl抑制下丘脑GT1—7神经元KiSS-1基因表达的机制研究  

AMP-activated protein kinase inhibits KISS-1 gene expression through SP1 in the hypothalamic GT1-7neurons

作　　者：温俊平[1] 胡雅婷[1] 刘春娥[1] 毕文凯[1] 黄惠彬[1] 梁继兴[1] 李连涛[1] 林丽香[1] 陈刚[1] 

机构地区：[1]福建医科大学省立临床医学院、福建省立医院内分泌科、福建省立医院北院、福建省老年医院内分泌科,350001

出　　处：《中华内分泌代谢杂志》2012年第9期754-757,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：基金项目：国家自然科学基金资助项目（30900510、81170774）;福建省自然科学基金资助项目（2011JOll27、2011J06012）;福建省医学创新课题（2011-CXB-2）

摘　　要：在GT1—7细胞用实时定量PCR检测腺苷酸活化的蛋白激酶（AMPK）对KiSS-1mRNA水平的影响．以报告基因技术检测KiSS-1基因启动子的活性，用Western印迹法检测AMPK对转录因子SPl蛋白表达及其在亚细胞中分布的影响。结果显示，AMPK能降低GT1-7细胞KiSS-1mRNA的表达水平，并能抑制KiSS-1基因的启动子活性；SP1能够增强KiSS-1基因的启动子活性；AMPK能够抑制SP1向细胞核的转位。提示AMPK可能通过抑制转录因子SP1的转位而降低KiSS-1基因的表达。The effect of AMP-activated protein kinase （AMPK） on KISS-1 mRNA levels was detected by realtime PCR in the hypothalamic GT1-7 neurons. The promoter activity of KISS-1 gene was detected by Dual- Luciferase Reporter Assay System. The effects of AMPK on the protein expression and subcellular distribution of SP1 were determined by Western blot. The results showed that AMPK reduced the mRNA expression and promoter activity of KISS-1 gene while SP1 increased the promoter activity of KiSS-1 gene. Besides, AMPK alse decreased the translocation of SP1. These results suggest that AMPK may inhibit the expression of KISS-1 gene by decreasing the translocation of SPI from cytoplasm to nucleus in the hypothalamus GT1-7 neurons.

关 键 词：KISS-1 腺苷酸活化的蛋白激酶 SP1 转位 

分 类 号：R363[医药卫生—病理学]