半制备液相色谱法分离制备油茶果皮多酚的优化条件  

Optimum condition of isolation and preparation of polyphenol in Camellia pericarp by semi-preparation HPLC

作　　者：赵清洁[1,2,3] 龙奇志[1,2,3] 钟海雁[1,2,3] 周波[1,2,3] 朱晓阳[1,2,3] 朱培[1,2,3] 

机构地区：[1]稻谷及副产物深加工国家工程实验室,湖南长沙410004 [2]粮油深加工与品质控制湖南省重点实验室,湖南长沙410004 [3]中南林业科技大学食品科学与工程学院,湖南长沙410004

出　　处：《经济林研究》2012年第2期73-77,共5页Non-wood Forest Research

基　　金：国家自然科学基金项目(31070612);湖南省自然科学基金项目(09JJ3042)

摘　　要：为了提高物质的制备量和分离度,采用半制备液相色谱法对油茶果皮多酚的制备条件进行了优化实验。结果表明,在用50%乙醇提取油茶果皮多酚的基础上,以半制备液相色谱法分离纯化油茶果皮多酚的优化条件如下:流动相A(水∶乙酸=100∶1)和B(乙腈),以2.5 mL/min的流速进行梯度洗脱,进样量为0.4 mL。实验结果还表明:按此方法制备所得单一多酚组分含量可达21.22 mg/g。这一实验结果证明了此法有效可靠。In order to improve preparation quantity and separation degree, the preparation condition of polyphenol in Camellia pericarp was optimized by semi-preparation HPLC. The results showed that after extracting polyphenol with 50% of alcohol, the optimization condition of isolating and purifying polyphenol by semi-preparation HPLC was as follows： gradient elution was carried on by mobile phase A （volume ratio of water to acetic acid was 100 ： 1） and B （acetonitrile） at 2.5 mL/min flow rate, the injection volume was 0.4 mL. The content of single polyphenol was 21.22 mg/ g through the preparation method, and it was effective and reliable.

关 键 词：油茶果皮 半制备液相色谱 多酚 乙醇 

分 类 号：S609.9[农业科学—园艺学] S794.2