原发性胆汁性肝硬化患者外周血T细胞microRNA表达谱变化  

MicroRNA profiling in T cells of peripheral blood mononuclear cell from patients with primary biliary cirrhosis

作　　者：钱琤 汪怀周[2] 范华杰[2] 谷明莉[2] 任传路[2] 邓安梅[2] 仲人前[3] 

机构地区：[1]解放军第一00医院检验科,苏州215007 [2]上海长海医院实验诊断科 [3]上海长征医院实验诊断科

出　　处：《中华医学杂志》2012年第32期2265-2267,共3页National Medical Journal of China

基　　金：国家自然科学基金（30972730、30772017）;上海市科委基金（09JCl405400、11JCl410902）

摘　　要：目的观察原发性胆汁性肝硬化（PBC）患者外周血T细胞中微小RNA（microRNA，miRNA）表达谱的变化。方法选取20例PBC患者，收集同期20份健康人外周血作对照。应用芯片技术筛选PBC患者外周血T细胞中表达差异的miRNA，并用实时定量PCR进行验证。结果与健康对照组相比，PBC患者外周血T细胞中有25个miRNA表达失调，其中有23个下调，仅有2个上调（均P〈0．05）。定量PCR显示，miR-346、miR-17-5p、miR-20a和miR-let-7b表达显著低于健康对照，而miR-451和miR-129表达显著高于健康对照，与芯片结果一致。结论PBC患者外周血T细胞miRNA表达与健康对照比较存在差异，这些异常表达的miRNA可能参与PBC的发病。Objective To explore the expression pattern of microRNA （miRNA） in T cells of peripheral blood mononuclear cell （PBMC） from patients with primary biliary cirrhosis （PBC）. Methods The expression profile of miRNA in T cells of PBMC was determined by microarray assay and validated by real-time quantitative reverse transcription-polymerase chain reaction （qRT-PCR）. Results In comparison with the healthy controls, 23 miRNA were down-regulated and 2 miRNA had a higher expression （ all P 〈 0. 05）. As revealed by qRT-PCR, the expressions of miR-346, miR-17-Sp, miR-20a and miR-let-Tb decreased obviously while miR-451 and miR-129 became up-regulated. The results were in agreement with those of microarray. Conclusions The PBG patients and healthy controls have significantly different expression profiles of microRNA in T cells of PBMC. The differential expression of microRNA may be involved in the pathogenesis of PBC.

关 键 词：肝硬化 胆汁性 微RNAS 自身免疫疾病 

分 类 号：R575.2[医药卫生—消化系统]