结缔组织生长因子-siRNA对糖尿病肾病大鼠肾组织中转化生长因子-β1及层黏连蛋白表达的影响  

Effect of connective tissue growth factor-siRNA on expression of TGF-β1 and fibronectin in renal tissue of diabetic nephropathy rat

作　　者：赵正林[1] 张杰[1] 袁晓环[2] 崔荣军[1] 王桂云[1] 金相赞 梁在夏 赵容杰[4] 

机构地区：[1]牡丹江医学院生物化学教研室,牡丹江157011 [2]牡丹江医学院医药研究中心,牡丹江157011 [3]韩国大邱韩医科大学韩医系,大邱706—828,韩国 [4]牡丹江医学院药理学教研室,牡丹江157011

出　　处：《解剖学杂志》2012年第4期451-454,共4页Chinese Journal of Anatomy

基　　金：黑龙江省教育厅回国留学人员科研基金（1154h24）

摘　　要：目的：探讨结缔组织生长因子（CTGF）si-RNA对糖尿病大鼠肾组织中转化生长因子-β（TGF-β1）及层黏连蛋白表达的抑制作用。方法：取健康雄性Wistar大鼠，随机分为正常对照组、糖尿病模型组、CTGF-siRNA隐性对照组、CTGF-siRNA实验组。腹腔注射1％链脲佐菌素（STZ）建模。特殊转染载体通过尾静脉注射CTGF-siRNA。免疫组织化学观察肾组织中TGF-β1，的表达，RT-PCR方法观察肾皮质中TGF-β1、层黏连蛋白mRNA表达的变化。结果：CTGF-siRNA实验组肾组织中TGF-β1。的表达减少，同时肾皮质中TGF-β1、层黏连蛋白mRNA表达也减少。CTGF-siRNA注射结束后第4周，尿蛋白明显降低。结论：CTGF-siRNA对糖尿病大鼠肾组织中TGF-β1、层黏连蛋白表达有抑制作用，缓解肾组织的纤维化作用，同时对糖尿病肾功能有明显改善作用。Objective：To investigate the effect of CTGF-siRNA on the expression of TGF-β1 and fibronectin in the renal tissue of rat with diabetes. Methods： Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin for consecutive three days. After the development of diabetes, the rats were treated with CTGF-siRNA for every two days for three times. Four weeks after the last dose of CTGF-siRNA, the rats were killed and the kidneys were removed. Immuno-histochemistry was done to observe the protein expression of TGF-β1 in the renal tissues and RT-PCR analysis was also done to examine the rnRNA expression of TGF-β1 and fibronectin in the renal cortex. In order to evaluate the renal function, 24 h urine protein was analyzed at day 7, 14, 21 and 28. Results：CTGF-siRNA significantly decreased the expression of TGF- β1 protein and mRNA in the renal tissues and also greatly reduced the fibronectin mRNA expression in the renal cortex Additionally, there was also significant reduction of urine protein at the 28th day. Conclusion：CTGF-SiRNA can inhibit the increased expression of TGF-β1 and fibronectin in the renal tissues, and also improve renal function in diabetic nephropathy rats.

关 键 词：结缔组织生长因子 SIRNA 糖尿病肾病 转化生长因子-Β1 层黏连蛋白 

分 类 号：R575.2[医药卫生—消化系统]