脑源性神经营养因子对急性高眼压大鼠视网膜突触囊泡素表达的影响  

Effect of brain-derived neurotrophic in retina following acute factor on expression of synaptophysin high intraocular pressure

作　　者：王慧[1] 罗佳[1] 周利红[1] 黄菊芳[1] 陈旦[1] 

机构地区：[1]中南大学湘雅医学院人体解剖学与神经生物学系,长沙410013

出　　处：《解剖学杂志》2012年第4期473-475,488,F0004,共5页Chinese Journal of Anatomy

基　　金：教育部博士点基金（20090162110019）;湖南省自然科学基金（10JJ4023）;中央高校基本科研业务费（2011QNZT128）;中南大学研究生教育创新工程项目（2010ssxt257）

摘　　要：目的：探讨脑源性神经营养因子（BDNF）在急性高眼压后大鼠视网膜突触可塑性中的作用。方法：SD大鼠随机分为正常对照组、假手术组、单纯高眼压组、溶媒预处理急性高眼压组、BDNF预处理急性高眼压组和BDNF抗体预处理急性高眼压组。预处理后2d，制作急性高眼压模型。动物存活1、3、7或14d后处死，冷冻切片行突触囊泡素（SYN）免疫组织化学检测。结果：SYN蛋白的免疫阳性产物主要分布于视网膜外网层和内网层，SYN在高眼压3d后在外网层分布增宽，7d时最宽，14d时又回复到与正常相似的模式；BDNF预处理后，在急性高眼压早期，SYN在视网膜外网层分布范围增宽的现象延后，至14d阳性产物分布面积达到高峰；BDNF抗体预处理后，SYN阳性产物分布范围增宽现象前移，在3d时达到高峰，但与正常水平差异无统计学意义，至7d和14dSYN回落。结论：急性高眼压后外源性BDNF干预延后了视网膜逆行性跨神经元突触改变的发生时间。Objective： To explore the effects of brain-derived neurotrophic factor （BDNF） on synaptic plasticity in rat retina following acute high intraocular pressure （HIOP）. Methods： SD rats were randomly divided into a normal control group, a sham group, a pure acute HIOP group, a vehicle pretreated HIOP group, a BDNF pretreated HIOP group and a BDNF antibody pretreated HIOP group. Two days after pretreatment, the HIOP animal model was made. The rats survived for 1 d, 3 d, 7 d, 14 d. Immunohistochemistry for synaptophysin （SYN） was determined with frozen sections. Results： SYN immunoreactivity was mainly distributed in the outer and inner plexiform layer （OPL and IPL） in the normal retina. From the third day after HIOP injury, the distribution of synaptophysin in the OPL was widened gradually and extended to the inner part of the outer nuclear layer （ONL） which reached the maximum distribution on the 7th day and returned similar to normal pattern on the 14th day. BDNF pretreatment generated delay of the widened distribution of SYN expression in the OPL and ONL. The area of SYN immunoreactivities increased markedly in the OPL and ONL at the 14th day after injury. In the BDNF antibody pretreatment group, contrarily, the widened distribution of SYN immunoreactivities was ahead of schedule to the 3^th day following acute HIOP and gradually fall next to normal pattern after 7d. Conclusion. BDNF may affect retinal synaptie plasticity by delaying the occurence of retrograde trans-synapse change following acute HIOP.

关 键 词：脑源性神经营养因子 高眼压 突触囊泡素 视网膜 大鼠 

分 类 号：R775[医药卫生—眼科]