Overexpression of Rice Sphingosine-1-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance  

作　　者：Huijuan Zhang Jing Zhai Jibo Mo Dayong Li Fengming Song 

机构地区：[1]State Key Laboratory for Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou,Zhejiang 310058,China

出　　处：《Journal of Integrative Plant Biology》2012年第9期652-662,共11页植物学报（英文版）

基　　金：supported by the National Key Project for Research on Transgenic Plants (2009ZX08001-017B and 2011ZX08009-003-001);the National Natural Science Foundation of China (No. 30971880);the National High-tech R&D Program of China (No. 2 012AA101504);the Program for Changjiang Scholars and Innovative Research Team in the University of the Ministry of Education of China (No. IRT0943)

摘　　要：Sphingolipids, including sphingosine-l-phosphate （SIP）, have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice （Oryza sativa） SlP lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid （ABA）, and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPLl-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPLl.overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.Sphingolipids, including sphingosine-l-phosphate （SIP）, have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice （Oryza sativa） SlP lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid （ABA）, and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPLl-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPLl.overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.

关 键 词：SPHINGOLIPIDS sphingosine-l-phosphate lyase （SPL） OsSPL1 salt stress oxidative stress. 

分 类 号：S572[农业科学—烟草工业] Q505[农业科学—作物学]