纤维素酶法提取土人参多糖的工艺研究  

Research on the Extraction Process of Polysaccharide from Talinum Crassifolium Using Cellulase

作　　者：农小英[1] 黄礼德[1] 郭立强[1] 潘廷啟[1] 颜祖弟[1] 张照平[1] 黄锁义[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《中国现代应用药学》2012年第9期802-804,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：2013年度广西自治区级大学生创新训练计划立项资助项目(QJCX201338);右江民族医学院民族医药协会资助项目(20110715)

摘　　要：目的优化纤维素酶法提取土人参多糖。方法通过单因素实验和正交实验,研究pH值、酶用量、酶解温度和提取时间对土人参多糖提取效果的影响。结果纤维素酶法提取的优化工艺条件为:pH值为6,每克药材酶用量为400 U,酶解温度50℃,提取时间3 h。在此条件下,土人参多糖的提取率平均为21.16%,RSD为0.14%。结论纤维素酶法提取土人参多糖效果省时高效。OBJECTIVE To opmtimize the extraction technique for total polysaccharide from Talinum Crassifolium. METHODS The single factor experiment and the orthogonal design were conducted to investigate the influence of pH, enzyme dosage, extraction temperature and extraction time on yield of Polysaccharide from Talinum Crassifolium. RESULTS The optimum conditions of cellulose enzyme were as follows： pH 6.0, enzyme dosage 400 U.g-l enzymes drug, temperature 50 ~C, extraction time 3 h, and extraction yield was 21.16, RSD was 0.14%. CONCLUSION Extraction polysaeeharide from Talinum Crassifolium by cellulose enzyme is a simple, quick and efficient method.

关 键 词：土人参 多糖 纤维素酶 正交设计 

分 类 号：R284.2[医药卫生—中药学]