OPA-FMOC柱前衍生反相高效液相测定糯米香茶叶片中的氨基酸  

Determination of Amino Acids in Strobilanthes tonkinensis Landau Leaves by OPA-FMOC Pre-column Derivatization and RP-HPLC

作　　者：徐飞[1,2] 谭乐和[1,2] 陈鹏[1,2] 朱红英[1,2] 胡荣锁[1,2] 张翠玲[1,2] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]农业部香辛饮料作物遗传资源利用重点实验室,海南万宁571533

出　　处：《热带作物学报》2012年第8期1482-1486,共5页Chinese Journal of Tropical Crops

基　　金：海南省工程技术中心专项(琼科函[2010]418号)

摘　　要：采用邻苯二甲醛(OPA)-9-芴基甲氧基羰酰氯(FMOC)柱前衍生反相高效液相色谱法测定糯米香茶叶片中氨基酸的含量。测定了5种肥料处理下同一部位及不同部位的叶片中氨基酸含量。结果表明:5种不同肥料处理下叶片中氨基酸含量存在较大差异,其中605有机肥处理下,糯米香茶底部叶片中的氨基酸含量最高。此研究为糯米香茶的种植栽培及食品研究开发提供了科学依据。The amino acids in Strobilanthes tonkinensis Landau leaves were determined by OPA-FMOC pre-column derivatization and RP-HPLC. The contents of the amino acids in the leaves under five kinds of fertilizer application were determined. The results showed the contents of the amino acids in the leaves had great difference under the five kinds of fertilizer application. The highest content of amino acids was in the bottom leaves of Strobilanthes tonkinensis Lindau with 605 organic fertilizer treatment. The study offers a scientific basis for the cultivation of Strobilanthes tonkinensis Landau and food research and development.

关 键 词：糯米香茶 氨基酸 柱前衍生 反相高相液相 

分 类 号：O657.7[理学—分析化学]