应用飞行时间质谱技术结合蛋白芯片筛选鼻咽低分化鳞癌患者血清肿瘤标志物的研究  

Combination of SELDI-TOF-MS with protein microarrays to screen serum biomarkers in patients with poorly differentiated nasopharyngeal squamous cell carcinoma

作　　者：李龄[1] 朱小东[1] 苏芳[1] 曲颂[1] 王琪[2] 李力[3] 张玮[2] 

机构地区：[1]广西医科大学附属肿瘤医院放疗科,南宁530021 [2]广西医科大学附属肿瘤医院实验研究部,南宁530021 [3]广西医科大学附属肿瘤医院妇瘤科,南宁530021

出　　处：《中国癌症防治杂志》2012年第3期214-217,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：吴阶平医学基金会临床科研专项资助基金(320.6750.06080);广西科学研究与技术开发计划课题(桂科攻0719006-2-23);广西研究生教育创新计划资助项目(2007105981002M33);广西大型仪器协作共用网测试补助项目(533-2007-112);(555-2007-134)

摘　　要：目的探讨筛选鼻咽低分化鳞癌血清肿瘤标志物的临床应用价值。方法应用表面增强激光解吸离子化飞行时间质谱(surface enhanced laser desorption/ionization time-of-flight mass spectrometry,SELDI-TOF-MS)及CM10型弱阳离子交换芯片对61例初治的鼻咽低分化鳞癌患者和72名健康人血清标本进行蛋白质指纹图谱测定,其中80例用于建模(40例鼻咽癌,40名健康人),53例用于盲法检测(21例鼻咽癌,32名健康人),用Biomarker Wizard3.01及Biomarker Pattern5.01分析软件对测得的数据进行处理及建立诊断模型。结果用建立的区分鼻咽低分化鳞癌与健康人的血清蛋白指纹图诊断模型进行盲法检测的准确性、敏感性和特异性分别为86.8%、81.0%和90.6%。其中Ⅰ、Ⅱ期鼻咽低分化鳞癌检出的准确性为73.7%,Ⅲ、Ⅳ期鼻咽低分化鳞癌检出的准确性为81.0%。结论用SELDI-TOF-MS技术与生物信息分析法筛选的血清肿瘤标志物对鼻咽低分化鳞癌的定性诊断提供了一条新途径。Objective To explore the clinical value of screening possible serum tumor markers in patients with poorly differentiated nasopharyngeal squamous cell carcinoma. Methods Surface-enhanced laser desorption ionization time-of-flight mass spectrometry （SELDI-TOF-MS）and a CM-10 protein chip were used to determine the serum proteomic patterns of 61 patients with poorly differen- tiated nasopbaryngeal squamous cell carcinoma before radiotherapy and 72 healthy subjects. Proteomic spectra of serum samples co- llected from 40 patients and 40 healthy controls were used as training sets.The validity of the classification trees were then challenged with blind test sets, which included another 21 patients with poorly differentiated nasopharyngeal squamous cell carcinoma and 32 healthy controls. Biomarker Wizard 3.01 and Biomarker Pattern 5.01 software were used together to analyze the data and develop di- agnostic models. Results The accuracy, sensitivity and specificity tested by blinded detection were 86.8%, 81.0% and 90.6%, respec- tively.The accuracy of 3 protein peaks（M4890, M5710 and M7780）was 73.7% for predicting stage I and II,and 81.0% for predicting stage Ill and IV of poorly differentiated nasopharyngeal squamous cell carcinoma. Conclusion SELDI-TOF-MS combined with bioin- formatics tools provides a new approach for qualitative diagnosis of poorly differentiated nasopbaryngeal squamous cell carcinoma.

关 键 词：鼻咽肿瘤 鼻咽低分化鳞癌 表面增强激光解吸离子化飞行时间质谱技术 肿瘤标志物 

分 类 号：R739.63[医药卫生—肿瘤]