RP-HPLC测定桑寄生及其寄主植物黄皮树盐酸小檗碱的含量  

Determination of Berberine in Taxilli Herba and Its Host Plants-Clausena lansium by RP-HPLC

作　　者：苏本伟[1] 李永华[1] 卢栋 朱开昕 裴河欢 赵明惠 李静 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]钦州市中医药研究所,钦州535000

出　　处：《世界科学技术-中医药现代化》2012年第4期1891-1894,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金面上项目(81173537):寄主植物对桑寄生药材质量影响评价研究;负责人:李永华

摘　　要：目的：测定桑寄生及其寄主植物中盐酸小檗碱的含量，研究寄主植物对桑寄生药材质量的影响。方法：采用甲醇一盐酸（100：1）溶液超声提取样品中的盐酸小檗碱，并采用RP—HPLC法进行含量测定，选用AgilentC18柱（250mmx4．6mm，5μm），流动相：乙腈：水：磷酸：三乙胺（72：28：0．01：0．05），流速：1．0mL·min-1，柱温：室温，检测波长：284nm。结果：不同寄主植物黄皮树及其桑寄生中盐酸小檗碱含量范围分别为0．14～7．58mg·g-1和0．11—1．35mg·g-1，桑寄生盐酸小檗碱含量最高可达到其寄主植物黄皮树盐酸小檗碱含量的132．10％。结论：寄主植物是影响桑寄生药材质量的关键，寄主植物不同桑寄生药材化学成分含量也不同。This study was aimed to determine contents of berberine in Taxilli Herba and its host plants Clausena lansium, and further to explore the effect of host plants on Taxilli Herba. Berberine was extracted with CH3OH- HCL （100：1） solution by ultrasound. The content of berberine was determined by RP-HPLC. Agilent Cls column （250 mm x 4.6 mm, 5 Ixm） was used for the determination, with a flow rate of 1.0 mL＇min-l and column tem- perature of 25℃. The mobile phase were acetonitrile-warter-phosphoric acid-triethylamine（72：28：0.01：0.05）. The detection wave length was 284 nm.The results showed that contents of berberine in Taxilli Herba and its host plants-Clausena lansium were 0.11 mg-g-1 - 1.35 mg＇g-1 and 0.14 mg＇g-1 - 7.58 mg＇g-1, respectively. The highest content of berberine in Taxilli Herba is as high as 132.10% as that in Clausena lansium. It was conclud- ed that host-plants exert a very important effect on the quality of Taxilli Herba. With different host plants, the content chemical composition in Taxilli Herba were different.

关 键 词：桑寄生 黄皮 盐酸小檗碱 RP—HPLC 紫外检测 

分 类 号：R286.0[医药卫生—中药学]