丛枝菌根真菌群落对白三叶草植株生物量磷吸收和土壤磷酸单酯酶活性的影响  

Effect of AMF Community on the Biomass of White Clover, Uptake of Phosphorus and Soil Phosphomonoesterase Activities

作　　者：钟敏[1] 黄益宗[1] 伍文[1] 隋立华[1] 贾炎[1] 王晓辉[1] 郝晓伟[1] 王晓英[1] 王幼珊 

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]北京市农林科学院植物营养与资源研究所,北京100089

出　　处：《农业环境科学学报》2012年第9期1770-1776,共7页Journal of Agro-Environment Science

基　　金：中国科学院知识创新工程重要方向资助项目(KSCX2-YW-N-41-05);国家自然科学基金面上项目资助项目(41071336)

摘　　要：丛枝菌根真菌(AMF)在土壤与植物的磷素循环中发挥着关键的作用。采用盆栽实验研究了丛枝菌根真菌群落对白三叶草植株生物量、磷吸收和土壤磷酸单酯酶活性的影响。结果表明,接种不同AMF群落均能显著地促进白三叶草植株的生长及其对磷素的吸收,提高根际土壤磷酸单酯酶的活性。Mnp处理中,白三叶草生物量最大,白三叶草总生物量、茎叶生物量和根系生物量分别比对照处理(-M)提高64.48%、61.48%和84.91%。不同菌根处理中,Mck处理显著地提高白三叶草磷吸收和土壤磷酸单酯酶活性,白三叶草磷吸收总量和茎叶磷吸收量分别比对照(-M)提高107.18%和91.91%,土壤碱性磷酸单酯酶和酸性磷酸单酯酶活性相对对照(-M)分别提高54.33%和138.43%。碱性磷酸单酯酶活性与AMF群落中的Acaullospora属孢子数呈显著的正相关关系,而酸性磷酸单酯酶活性则主要受Paraglomus属孢子数的影响。说明接种AMF群落可显著地影响土壤的磷酸单酯酶活性,从而影响白三叶草的生长及其对磷素的吸收。Arbuscular mycorrhizal fung（iAMF）play a crucial role in phosphorus circulation of plants and soil. Effects of different AMF com- munities upon white clover growth, uptake of phosphorus and soil phosphomonoesterase activities were performed by pot experiments. The results indicated that soil phosphomonoesterase activities, uptake of phosphorus and white clover biomass were enhanced significantly in each treatment with AMF inoculation. Among these eight treatments, biomass of plant tissue was highest in the Mnp treatment. In this treatment, plant total biomass, shoot biomass and root biomass were highest, which were 64.48%, 61.48% and 84.91% higher than control treatmen（t-M） respectively. Each AMF treatment induced positive effect on the uptake of phosphorus and phosphomonoesterase activity. The total phosphorus and shoot phosphorus of white clover in Mck treatment were 107.18% and 91.91% higher than control treatment respectively. Activities of alkaline phosphatase and acid phosphatase in Mck reached were separately increased by 54.33% and 138.43% compared with those in the control treatment. Pearson correlation analysis between AMF genus and phosphomonoesterase activities showed that alkaline phosphomonoesterase activities were positive relationship with abundance of Acaullospora while acid phosphomonoesterase were positive relationship with abundance of Paraglomus. Inoculations of AMF community could significantly influenced white clover growth, uptake of phosphorus and phosphomonoesterase activities in soil.

关 键 词：丛枝菌根真菌群落 磷酸单酯酶 磷 白三叶草 

分 类 号：X172[环境科学与工程—环境科学]