银杏内生真菌菌株R2的鉴定及其抑菌活性  

Identification of Endophytic Fungi Strain R2 from Ginkgo biloba and Its Antimicrobial Activity

作　　者：汪涯[1] 李希茜[2] 张志斌[3] 颜日明[1,3] 曾庆桂[3] 朱笃[1,3] 

机构地区：[1]宜春学院江西省天然药物活性成分研究重点实验室,江西宜春336000 [2]江西中医学院科技学院,江西南昌330025 [3]江西师范大学生命科学学院江西省亚热带植物资源保护与利用重点实验室,江西南昌330022

出　　处：《江西农业大学学报》2012年第4期718-723,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省亚热带植物资源保护与利用重点实验室开放基金资助(YRD201203)

摘　　要：以金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)和枯草芽孢杆菌(Bacillus subtilis)3种微生物为指示菌,采用杯碟法对分离自药用植物银杏(Ginkgo biloba L.)的36株内生真菌进行抗菌活性检测。并通过形态学特征观察和ITS-rDNA序列系统发育分析对强抑菌活性菌株进行鉴定。结果发现,菌株L1、L4、L5、L7、T2、T3、R1和R2等8株内生真菌的发酵粗提物具有抑菌活性,其中以内生真菌R2的抑菌活性最高,抑菌圈直径分别为20 mm、20 mm和21 mm。结合形态学特征和ITS-rDNA序列分析确定菌株R2为尖孢枝孢菌(Cladosporium oxysporum)。The antimicrobial activity plant Ginkgo biloba were detected by the of 35 endophytic fungi isolated from traditional Chinese medicinal method of cylinder plate test with Staphylococcus aureus, Escherichia coli and Bacillus subtilis as indicator microorganisms. Observation on the morphological characters and internal transcribed spacers ribosomal DNA （ITS-rDNA） sequence analysis were conducted to identify the strain. The results showed that the extracts of 8 isolated entophytic fungi strain L1, L4, [5, L7, T2, T3, R1 and R2 had antibiotic activity against some indicator microorganisms. Especially the extract of strain R2 obviously exhibited strongest antibiotic activity against those indicator microorganisms, the inhibition zones diameters were 20 mm, 20 mm and 21 mm respectively. Based on the morphological characters and ITS-rDNA sequence analysis, the endophytic fungi strain R2 was identified as Cladosporium oxysporum.

关 键 词：银杏 内生真菌 抑菌活性 鉴定 

分 类 号：S792.95[农业科学—林木遗传育种]