离子色谱-积分脉冲安培法测定烟草中18种游离氨基酸  

Determination of 18 Free Amino Acids in Tobacco with High Performance Anion-exchange Chromatography with Integrated Pulsed Amperometric Method

作　　者：于宏晓[1] 徐海涛[1] 马强[1] 吕健[1] 张晋[1] 岳勇[1] 赵曰利 肖协忠[1] 

机构地区：[1]山东中烟工业有限责任公司技术中心,青岛266101

出　　处：《中国烟草科学》2012年第4期51-54,共4页Chinese Tobacco Science

基　　金：山东中烟工业公司资助项目(110000038)

摘　　要：采用离子色谱-积分脉冲安培法同时测定了烟草中18种游离氨基酸的含量。结果表明,该方法同时检测烟草中18种游离氨基酸的平均回收率为80%～115%,最低检出限为0.03～0.75μg/mL,线性相关系数为0.9900～0.9992。该方法成功对比分析测定了烤烟、白肋烟和香料烟3种不同类型单料烟以及国内外不同风格卷烟中18种游离氨基酸的含量。18 of free amino acids in tobacco were simultaneously determined by high performance anion-exchange chromatography with integrated pulsed amperometric detection method. The results showed that the recoveries for 18 amino acids were in the range of 80%-115 %, the detection limits for 18 amino acids were 0.03-0.75 ~tg/mL and the calibration graphs of peak area for the analyses were linear over about three orders of magnitude with a correlation coefficient of 0.9900-0.9992. The method was applied to determine 18 free amino acids in tobacco with satisfactory results. The method was employed to determine 18 free amino acids in flue-cured tobacco, burley tobacco and oriental tobacco. The method was also employed to determine successfully 18 free amino acids of different brands of domestic cigarettes and overseas cigarettes.

关 键 词：离子色谱 积分脉冲安培法 游离氨基酸 卷烟 

分 类 号：S572[农业科学—烟草工业]