幽门螺杆菌上调Bcl-xL基因的表达促进胃癌BGC-823细胞的增殖  

Upregulation of Bcl-xL mRNA Cellular Expression by Helicobacter pylori Leads to the Proliferation of Gastric Cancer BGC-823 Cells

作　　者：吴莺[1] 孙海波[2] 艾宽宽[2] 李翔[3] 蒋晓猛[1] 周红[4] 

机构地区：[1]江苏大学附属医院消化科,江苏省镇江市212001 [2]江苏大学 [3]东台市人民医院消化科 [4]江苏大学医学基础与医学技术学院

出　　处：《中国肿瘤临床》2012年第18期1342-1345,共4页Chinese Journal of Clinical Oncology

基　　金：江苏省研究生培养创新计划基金(编号:1221270009);镇江市科技计划项目(编号:SH2007025)资助~~

摘　　要：目的:探讨幽门螺杆菌(H.pylori)对胃癌细胞BGC-823 Bcl-xL基因表达的影响。方法:分别用东、西方型H.Pylori裂解液处理胃癌细胞,MTT法检测细胞增殖情况,荧光定量PCR法检测细胞Bcl-xL基因mRNA表达水平的变化;Bcl-xL短发夹RNA(short hairpin,shRNA)质粒沉默Bcl-xL基因,MTT法检测胃癌细胞增殖能力。结果:H.pylori裂解液处理胃癌细胞24h后,与对照组相比,处理组均出现细胞的增殖(P均<0.01),并且东亚型处理组的增殖作用比西方型处理组更明显(P<0.01);Bcl-xL基因的mRNA表达水平也均出现上调(P均<0.01),东亚型处理组的上调水平比西方型处理组更明显(P<0.01);Bcl-xL shRNA质粒转染胃癌细胞BGC-823后,与对照组、Bcl-xL shRNA阴性对照组相比,细胞增殖受到抑制。结论:H.pylori的生物活性物质通过上调Bcl-xL基因的表达促进胃癌细胞BGC-823的增殖,东亚型H.pylori的生物活性作用比西方型强。Bcl-xL shRNA质粒在一定程度上可抑制胃癌细胞BGC-823的增殖。Abstract Objective： This study aims to investigate the effect of Helicobacter pylori on the expression of the Bcl-xL gene and its potential effect on human gastric cancer BGC-823 cell lines. Methods： Human gastric cancer BGC-823 cell lines were treated with extracts from East Asian-type andWestern-type H. pylori. Cell proliferation was evaluated by MTT assay. The mRNA level of Bcl-xL was detected by real-time quantitative PCR. The Bcl-xL-mediated RNA interference technique was employed to inhibit Bcl-xL gene expression and BGC-823 cell proliferation. The mRNA level, Bcl-xL protein expression, and inhibitory percentage of BGC-823 cells were detected by RT-PCR, western blot, and MTT assay, respectively. Results： The proliferation of BGC-823 cells treated with H. pylori extract was observed after 24 hours （ P 〈 0.01 ） in relation to the control group. The enhanced cellular proliferation in the East Asian type was higher than that in the Western type （ P 〈 0.01 ）. The expression of Bcl-xL mRNA in the groups treated with H. pylori extract was significantly elevated （all P 〈 0.01 ） compared with the control group. Statistical difference in Bcl-xL mRNA expression was also found between the East Asian type group and theWestern type group （ P 〈 0.01 ）. Bcl-xL shRNA significantly reduced Bcl-xL mRNA and protein expressions as well as BGC-823 proliferation. Conclusion： The biologically active elements in H. pylori extract induced the proliferation of gastric epithelial cells by upregulating the expression of Bcl-xL mRNA in human gastric cancer cells. The East Asian-type H. pylori showed stronger influence on cell proliferation and Bcl-xL mRNA expression compared with the Western type. This result implies that the East Asian-type H. pylori had much more biological activity than the Western type. Moreover, Bcl-xL shRNA inhibited Bcl-xL expression and BGC-823 cell proliferation.

关 键 词：细胞增殖 BCL-XL 幽门螺杆菌 BCL-XL shRNA质粒 细胞增殖 CagA蛋白分型 BCL-XL BCL-XL shRNA质粒 CagA蛋白分型 

分 类 号：R735.2[医药卫生—肿瘤]