人疱疹病毒8型ORF26基因亚型与鳞状细胞癌的相关性研究  

Characterization of human herpesvirus-8 genotypes in squamous cell carcinoma by using the polymor- phisms in open reading frame 26 gene

作　　者：李桂寅[1] 冯燕艳[1] 张德志[1] 吴秀娟[1] 刘建勇[1] 普雄明[1] 

机构地区：[1]新疆维吾尔自治区人民医院皮肤性病科,乌鲁木齐830001

出　　处：《中华皮肤科杂志》2012年第10期750-751,共2页Chinese Journal of Dermatology

基　　金：新疆维吾尔自治区自然科学基金（2009211A24）

摘　　要：目的探讨新疆鳞状细胞癌（简称鳞癌）患者感染人疱疹病毒8型（HHV-8）ORF26基因亚型。方法巢式PCR扩增41例皮肤鳞癌、46例食管鳞癌石蜡包埋组织标本中HHV-8ORF26基因，对PCR阳性产物进行双向测序，使用Dnastar软件、ClustalW软件和Phylip软件包对测序结果进行系统发生学分析，从而确定HHV-8ORF26基因亚型。运用SPSS17．0进行统计学分析。结果41例皮肤鳞癌组织标本9例HHV-8感染为阳性，阳性率为21．95％；46例食管鳞癌10例为阳性，阳性率为21．74％。新疆皮肤鳞癌患者感染HHV-8ORF26亚型7例为A亚型，2例为c亚型；食管鳞癌感染HHV-8ORF26亚型7例为A亚型，3例为C亚型。结论新疆鳞癌患者感染HHV-8ORF26亚型属于A亚型和C亚型，A亚型多于c亚型，亚型分布与鳞癌患者的发病部位无关。Objective To analyze the genotypes of human herpesvirus 8 （HHV-8） by using the polymorphisms in open reading frame （ORF） 26 gene in patients with squamous cell carcinoma （SCC）in Xinjiang Uygur Autonomous Region. Methods DNA was extracted from paraffin-embeded tissue specimens from 41 patients with skin SCC and 46 patients with esophagus SCC, and subjected to nested-PCR for the amplification of the ORF26 gene of HHV-8 followed by bidirectional sequencing. Phylogenetic analysis was carried out to determine the genotype of HH^-8 by using the DNASTAR software, Clustal W program, and PHYLIP package. The data were analyzed by SPSS 17.0 software. Results HHV-8 DNA was detected in 9 （21.95%） of 41 skin SCC specimens and 10 （21.74%） of 46 esophagus SCC specimens （A3 = 0.09, P 〉 0.05）. As phylogenetie analysis showed, 7 HHV-8 isolates from skin SCC specimens belonged to ORF26 subtype A, 2 subtype C; 7 HHV-8 isolates from esophagus SCC specimens belonged to ORF26 subtype A, and 3 subtype C. Conclusions ORF26 subtype A and C are the predominate genotypes of HHV in patients with SCC in Xinjiang Uygur Autonomous Region, with the prevalence of subtype A higher than that of subtype C. The distribution of HHV subtypes seems unrelated to the location of SCC.

关 键 词：人疱疹病毒8型 基因亚型 鳞状细胞癌 巢式PCR扩增 相关性 石蜡包埋组织标本 统计学分析 皮肤鳞癌 

分 类 号：R73[医药卫生—肿瘤]