人前列腺癌肿瘤干细胞12-LOX表达的初步研究  

Expression of arachidonate 12-lipoxygenase in prostate cancer stem cells

作　　者：殷波[1] 石武 王鹏[1] 刘岗[1] 李明[1] 宋永胜[1] 

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004 [2]营口经济技术开发区中心医院泌尿外科,辽宁营口115007

出　　处：《中华肿瘤防治杂志》2012年第16期1216-1220,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：辽宁省教育厅高等学校科研项目(2009A735)

摘　　要：目的:研究花生四烯酸12-脂氧化酶(12-LOX)在人前列腺癌肿瘤干细胞中的表达情况,并探讨其意义。方法:采用反转录聚合酶链反应(RT-PCR)技术和蛋白质印迹法,检测12-LOX在人前列腺癌细胞系DU 145细胞和从其中分离出来的DU 145侧群细胞中的表达情况;采用免疫荧光4通道激光扫描共聚焦显微镜技术,检测12-LOX在7例前列腺癌组织肿瘤干细胞和10例正常前列腺组织干细胞中的表达情况。结果:在细胞水平,应用RT-PCR技术和蛋白质印迹法分别证实,12-LOX mRNA和12-LOX蛋白在具有肿瘤干细胞特性DU 145侧群细胞中的表达水平明显高于对应的母系DU 145细胞;在组织水平,应用免疫荧光4通道激光扫描共焦显微镜检测证实,12-LOX蛋白在所有7例前列腺癌组织的肿瘤干细胞中均为阳性表达,而在所有10例正常前列腺组织的前列腺干细胞中均无表达。结论:12-LOX在人前列腺癌的肿瘤干细胞中有特异性的高表达,从而有望成为前列腺癌肿瘤干细胞一个新的、特异性的标志,并进一步有望成为前列腺癌肿瘤干细胞一个新的治疗靶位。OBJECTIVE： To examine the expression of arachidonate 12-1ipoxygenase （12-LOX） in human prostate cancer stem cells to test that can it serve as a unique marker and therapeutic target for prostate cancer stem cells. METH- ODS： The expression of 12-LOX was detected in DU 145 cells and DU 145 side population cells by reverse transcription polymerase chain reaction （RT-PCR） and Western blot. Paraffin-section based immunofluorescent 4-channel confocal mi- croscopy was applied to detect the expression of 12-LOX in prostate cancer stem cells and normal prostate epithelial stem cells identified in tissue samples. RESULTS： At cell level we detected a strong up-regulation of 12-LOX mRNA and 12-LOX protein in cancer stern cell-like DU 145 side populatin cells compared to the parental DU 145 cells by RT-PCR and Western blot approaches. At tissue level we also detected that 12-LOX protein was overexpressed in prostate cancer stem cells in 7 cases of human prostate cancer tissue samples by immunofluorescent 4-channel confocal microscopy. No 12-LOX was detected in normal prostate epithelial stem cells in 10 cases of normal prostate tissue samples. CONCLUSION： These multiple lines of evidence validate that 12 LOX has a specific overexpression in human prostate cancer stem cells and sup port the possibility that 12-LOX may serve as a unique marker and therapeutic target for prostate cancer stem cells.

关 键 词：前列腺肿瘤 12-LOX 干细胞 肿瘤干细胞 侧群 印迹法 蛋白质 

分 类 号：R737.25[医药卫生—肿瘤]