湖南省1979-2010年狂犬病流行特征及其毒株遗传关系分析  

Analysis of the epidemiological characteristics and phylogeography of rabies prevalent in Hunan Province,1979-2010

作　　者：蔡亮[1] 高立冬[1] 胡世雄[1] 刘运芝[1] 刘富强[1] 申辛欣[2] 陶晓燕[2] 李浩[2] 刘佳惠[1] 王世清[1] 曾舸[1] 唐青[2] 张红[1] 

机构地区：[1]湖南省疾病预防控制中心 湖南省微生物分子生物学重点实验室,湖南长沙410005 [2]中国疾病预防控制中心病毒病预防控制所 病毒基因工程国家重点实验室,北京102206

出　　处：《中华疾病控制杂志》2012年第9期774-777,共4页Chinese Journal of Disease Control & Prevention

基　　金：湖南省卫生厅科研基金(A2007008;B2009-088)

摘　　要：目的分析1979-2010年湖南省狂犬病流行特征及分离病毒N基因特征,探讨疫情波动影响因素及病毒进化情况。方法采用回顾性与描述性流行病学方法对湖南省32年来,狂犬病疫情数据进行统计分析;利用PubMed-Entrez Search程序建立GenBank、EMBL报道的湖南省境内分离的狂犬病毒N基因序列本地数据库,应用生物信息学方法分析病毒N基因特征与变异情况,参考贵州与广西2个高发省份分离的部分狂犬病毒及国内疫苗株CTN-1-V N基因序列,运用Clustal W 1.83软件进行多重序列比对,运用MEGA 5.0.4软件构建N基因系统进化树,分析湖南省分离毒株与贵州、广西分离株亲缘关系及病毒遗传特征差异。结果 32年来,湖南省累计报告狂犬病病例12 576例,总发病率0.69/10万;全省124个县(市、区)均有疫情报告;病例集中于湘南与湘中地区;7～10月份发病数较多(43.23%);农民所占比例最大(64.35%)。58株毒株N基因全序列平均GC含量为44.49%,AT含量为55.51%,平均单链分子量为409.7 KDa;氨基酸组成含量最高的为亮氨酸(Leu),含量最低的为色氨酸(Trp);58株毒株与贵州、广西及国内疫苗株有较近亲缘关系;58株病毒全部属于狂犬病毒基因I型。结论 32年来,湖南省狂犬病疫情存在较大波动;所分离的58株毒株遗传较稳定,未发生关键性变异。Objective To analyze the epidemic and genetic characteristics of rabies prevalent in Hunan Province, 1979-2010. Methods Retrospective and descriptive epidemiology were applied to analyze the dynamic distribution in space, time and crowd on the basis of the rabies data collected in the recent 32 years. A local rabies N gene database of Hunan Province was established according to the reporting of GenBank and EMBL by PubMed-Entrez Search software, the characteristics and variation of N gene of all selected sequences were analyzed by bioinformatics methods. The antigenic and genetic of the virus N gene was analyzed after the phylogenetic tree was constructed by Clustal W 1.83 and MEGA 5.0.4 software. Results There were 12 576 rabies cases reported in the recent 32 years in Hunan Province and the total mortality rate was about 0.69/100 000, which scattered in the whole province including 124 counties. Most of the cases were centralized in the south and central areas of Hunan Province and nearly 43.23% of the cases occurred from July to October, 64.35% of the cases were farmers. The average content of GC and AT was respectively 44.49% and 55.51% of the 58 strains and the average single strand molecular weight was about 409.7 KDa. The highest content of amino acid component was Leu, while the lowest content was Trp. All of Hunan strains had a relatively genetic relationship to the Guizhou and Guangxi strains and 58 strains isolated in Hunan were belonged to rabies gene type I after the phylogenetic tree was successfully built. Conclusions The rabies epidemic situation has a large fluctuation in recent 32 years in Hunan Province, the 58 isolated strains are quite stabilize and have no critical variation.

关 键 词：狂犬病 流行病学 N基因 计算生物学 

分 类 号：R181.1[医药卫生—流行病学] R512.2[医药卫生—公共卫生与预防医学]