ILK反义寡核苷酸对人上皮性卵巢癌裸鼠皮下移植瘤的治疗作用  

Therapeutic effect of ILK-ASODN on subcutaneous xenograft of human epithelial ovarian cancer in nude mice

作　　者：李奇[1] 李琛[2] 张云艳[1] 孙静[1] 刘艳雨[1] 

机构地区：[1]哈尔滨医科大学附属第三医院妇放科,黑龙江哈尔滨150081 [2]黑龙江省医院皮肤科,黑龙江哈尔滨150036

出　　处：《哈尔滨医科大学学报》2012年第4期346-349,353,共5页Journal of Harbin Medical University

基　　金：黑龙江省青年科学基金项目(QC2010067)

摘　　要：目的探讨整合素连接激酶(integrin linked kinase,ILK)反义寡核苷酸(antisense oligonucleoti-de,ASODN)对人卵巢癌裸鼠皮下移植瘤的治疗作用。方法将人卵巢癌HO-8910细胞株接种于裸鼠皮下,建立人卵巢癌裸鼠模型,并将裸鼠随机分为对照组和治疗组,每次腹腔注射治疗后称量裸鼠体重、肿瘤体积及重量,并计算抑瘤率。同时,分别用脂质体和脂质体-ILK-ASODN转染HO-8910细胞,用WST-1法检测转染后两组细胞体外的增殖情况,用流式细胞术检测细胞凋亡情况。结果治疗组裸鼠肿瘤体积及重量增长速度受到明显抑制,与对照组比较差异有统计学意义(P<0.01)。转染了ILK-ASODN的治疗组细胞增殖受到显著抑制,凋亡率明显增高,与对照组比较差异均具有极显著意义(P<0.01)。结论 ILK-ASODN在体外能抑制细胞生长、促进细胞凋亡;在体内能明显地抑制肿瘤生长,对人卵巢癌裸鼠移植瘤具有一定的治疗作用。Objective To explore the therapeutic effects of integrin-linked kinase antisense oli- gonucleotides （ILK-ASODN） on xenograft of human epithelial ovarian cancer in nude mice. Methods Human ovarian cancer H0-8910 cell lines were subcutaneously inoculated in nude mice to establish the model. Tile mice were then randomly divided into control and treatment groups. After each treatment of intraperitoneal injection, the weights, tumor sizes and the in- hibitory rates of nude mice were recorded. In the mean time, liposomes and liposome-ILK- ASODN were transfected to HO-8910 cell lines of control and treatment groups, respectively. WST-1 assay was used to measure the cell proliferation of the two groups after transfection. Cell apoptosis was detected by flow cytometry. Results The tumor volume and weight growth of the nude mice in the treatment group were significantly lower in comparison with those in the con- trol group （ P 〈 0.01 ）. Cell proliferation after transfection of ILK-ASODN in the treatment group was significantly inhibited, and apoptosis was signlhcantly higher （ P〈0.01 ）. Conctu- sion ILK-ASODN in vitro can inhibit cell growth and promote apoptosis; ,significantly inhibit tumor growth lial ovarian cancer in indicating a therapeutic effect on subcutaneous xenograft of human epithe- nude mice.

关 键 词：ILK ASODN 转染 基因治疗 裸鼠 移植瘤 

分 类 号：R737.31[医药卫生—肿瘤]