环氧化酶2在铝负荷致大鼠海马神经元损伤中的作用  

Effect of cyclooxygenase 2 on hippocampal neuronal damage induced by aluminum in rats

作　　者：吴柯[1] 万立华[2] 谢灵瑶[1] 杨俊卿 

机构地区：[1]重庆医科大学药学院重庆市生物化学与分子药理学重点实验室 [2]重庆医科大学基础医学院法医学教研室,重庆400016

出　　处：《中国病理生理杂志》2012年第9期1695-1698,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30672211)

摘　　要：目的:研究环氧化酶2(COX-2)在铝盐致大鼠海马神经元损伤中的作用。方法:原代海马神经元培养7 d,予以铝盐负荷(终浓度200μmol.L-1)建立大鼠海马神经元损伤模型,以COX-2特异性干扰腺病毒(MOI=100)转染神经元,Western blotting检测COX-2蛋白表达水平,酶化学法检测海马神经元超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、乳酸脱氢酶(LDH)漏出率及MTT值,倒置荧光显微镜观察海马神经元病理形态变化。结果:COX-2特异性干扰腺病毒转染的神经元COX-2蛋白表达下降,SOD活性、MDA含量、LDH漏出率、MTT值以及大鼠海马神经元的形态和结构未见明显异常变化,但其明显提高了铝盐负荷基础上的海马神经元存活率和SOD活性,显著降低了LDH漏出率和MDA含量,并改善了铝盐负荷后神经元的病理学形态。结论:海马神经元COX-2表达的适度沉默,不会影响海马神经元的形态和功能,但对于铝负荷导致的神经元损伤有保护作用。AIM：To study the relationship between cyclooxygenase 2（COX-2） and the damage of hippocampal neurons by aluminum overload in rats. METHODS：Newborn SD rats（less than 24 h） were used to establish the model of primary culture of hippocampal neurons. The neurons were treated with aluminum at concentration of 200 μmol/L. The techniques of RNA interference（RNAi） and cell transfection were used to study the role of COX-2 in hippocampal neuron. Following RNAi by cell transfection, Western blotting analysis was used to determine the protein expression of COX-2. Cell growth was assayed by the method of MTT. The pathological changes of the neurons were observed by fluorescence labeling. The activity of superoxide dismutase（SOD） and lactate dehydrogenase（LDH）, and the content of malondialdehyde（MDA） were detected for evaluating cell damage. RESULTS：COX-2 RNAi by cell transfection significantly decreased the protein expression of COX-2 without changing the neuronal pathomorphology, cell viability, SOD activity and MDA content. However, it obviously improved livability and SOD activity of the hippocampal neurons, which were aluminum-overloaded. Inhibition of COX-2 expression also reduced the leakage of LDH and the content of MDA, and ameliorated the pathological changes in neurons. CONCLUSION：Moderate silence of COX-2 expression not only significantly affects the morphological changes and physiological functions of hippocampal neurons, but also prevents the neurons from aluminum-induced damages.

关 键 词：环氧化酶2 神经元损伤 铝 

分 类 号：R363[医药卫生—病理学]