肠炎沙门氏菌SEF14菌毛亚单位sefA和sefD基因缺失株的致病性研究  

Pathogenicity of the Salmonella enteritidis with SEF14 fimbriae subunit sefA and sefD gene deletions

作　　者：朱春红[1] 孟霞[2] 姚丰华[2] 胡艳[1] 束婧婷[1] 朱国强[2] 

机构地区：[1]江苏省家禽科学研究所,江苏扬州225125 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《中国预防兽医学报》2012年第10期786-789,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31101833;31101826);江苏省自然科学基金(SBK201122944);江苏省属高校自然科学重大基础研究项目(08KJA230002);江苏高校优势学科建设工程资助项目;国家863计划(2011AA100301)

摘　　要：为研究SEF14菌毛在肠炎沙门氏菌中的致病作用,本实验将SEF14菌毛亚单位编码基因缺失的肠炎沙门氏菌突变株50336△sefA和50336△sefD分别接种6周龄BALB/c小鼠和1日龄清远麻鸡,比较它们与其亲本菌株50336的致病性差异。结果显示,亲本菌株50336以及突变株50336△sefA、50336△sefD对小鼠的半数致死量分别为19.95 cfu、7.94 cfu和7.94 cfu,表明两个突变株对小鼠的致病性显著增强;而且对雏鸡的致病性结果也显示,突变株50336△sefA致病力较强,其感染雏鸡组死亡率为15%,而亲本菌株组死亡率为8.6%;另外50336△sefA和50336△sefD感染组7日龄时平均增重分别为15.38 g和18.39 g,亲本菌株组为19.06 g,对照组为26.43 g。研究结果表明SEF14菌毛亚单位基因缺失对肠炎沙门氏菌的致病性具有增强作用。To investigate the pathogenicity of the SEF14 fimbriae in Salmonella enteritidis, the pathogenicity of mutant strains 50336△sefA and 50336△sefD was compared with the parent strain 50336 in 6-week-old BALB/c mice and 1 day-old Qingyuan chickens via subcutaneous inoculation. The results showed that the LDs0 of the three strains were 19.95, 7.94 and 7.94 cfu in 6-week-old BALB/c mice, respectively. It suggested the virulence of S. enteritidis increased when injected with mutant strains 50336△sefA or 50336 △sefD. The results also indicated that they were highly heterogeneous in their ability to cause death in l-day-chickens, the mortality of 50336 and 50336/ksefA groups were 8.6%, 15%, respectively. Furthermore, the chick average weight-gain at 7-day-old in control group was 26.43 g, and 50336 group was 19.06 g, while groups of 50336△sefA and 50336△sefD were 15.38 g and 18.39 g, respectively. This suggested that the virulence of 50336△sefA was increased in chicken.

关 键 词：肠炎沙门氏菌 SEF14菌毛 半数致死量 

分 类 号：S852.61[农业科学—基础兽医学]