准好氧填埋场垃圾样品中甲烷氧化菌的群落多样性分析  

Community analysis of methane oxidizing bacteria in municipal solid waste semi-aerobic landfill

作　　者：张维[1] 岳波[2] 黄启飞[2] 黄泽春[2] 

机构地区：[1]长安大学杂志社,陕西西安710064 [2]中国环境科学研究院固体废物污染控制技术研究所,北京100012

出　　处：《生态环境学报》2012年第8期1462-1467,共6页Ecology and Environmental Sciences

基　　金：国家自然科学基金(No.50908220);中国科学院战略性先导科技专项(No.XDA05020601)

摘　　要：为了探讨准好氧垃圾填埋场中的甲烷氧化机理,本研究利用RealTime-PCR和PCR-DGGE技术对其中的甲烷氧化菌分别进行了定量分析和群落多样性分析。研究结果表明,PCR-DGGE技术可以用于垃圾样品中甲烷氧化菌的微生物多样性分析;在距导气管0、2.5、5.0、10.0和15.0 m处垃圾样品中甲烷氧化菌的数量分别为1.13×109、2.22×108、2.38×108、2.20×107和5.21×106g-1,呈现逐渐降低的趋势;垃圾样品中甲烷氧化菌的丰富度和香侬多样性指数依次为:0 m>2.5 m>5.0 m=10.0m>15.0 m,此外,垃圾样品中甲烷氧化菌可归为:Methylocaldum(甲基暖菌属)、Methylobacterium(甲基杆菌属)和Methylocystis(甲基孢囊菌属),且优势菌种为Type I型Methylocaldum。In order to probe the methane oxidation mechanism in municipal solid waste （MSW） semi-aerobic landfills, real-time PCR and PCR-DGGE technologies have been used to determine methanotrophs community composition and abundance in semi-aerobic landfill waste. The results showed that the real-time PCR and DGGE technologies were effective methods to analyze methanotrophic comunities and quantity. The methanotrophic numbers were 1.13×10^9, 2.22×10^8, 2.38×10^8, 2.20×10^7 and 5.21×10^6 per gram of dry waste samples far from the gas pipe of 0, 2.5, 5.0, 10.0 and 15.0 m in distance respectively, which revealed a gradual decrease with increasing distance from the air pipe. The values of sequence of Genotypic richness（S ） are 0 m 〉 2.5 m 〉 5.0 m = 10.0 m 〉 15.0 m, Based on the sequences and phylogenetic analysis, methanotrophic communities in the tests could be classified into three groups： Methylocaldum , Methylobacter, Methylocystis, and the first group was the predominant organism.

关 键 词：准好氧填埋场 甲烷氧化菌 定量PCR DGGE 多样性 

分 类 号：X172[环境科学与工程—环境科学]