紫杉醇与白藜芦醇联合用药对人肝癌细胞HepG-2活性的影响  

Research about the Joint Effect of Taxol and Resveratrol on Human Liver Cancer HepG-2

作　　者：耿文峰[1] 伍春莲[1,2] 陈杨琼[1] 张思楠[1] 孙警辉[1] 

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009 [2]三峡库区生态环境与生物资源省部共建国家重点实验室,重庆北培400715

出　　处：《西华师范大学学报（自然科学版）》2012年第3期261-264,269,共5页Journal of China West Normal University(Natural Sciences)

基　　金：三峡库区生态环境与生物资源省部共建重点实验室开放课题基金项目(SKL-2011-05)

摘　　要：作为两种较常见的抗癌药物,紫杉醇与白藜芦醇在临床试验中均证实具有良好的抗癌效果.本文意在研究紫杉醇与白藜芦醇单独及联合用药时对人肝癌细胞HepG-2活性的影响,探索两种药物联合应用时的药效变化.实验中通过CCK-8法测定了不同浓度的紫杉醇和白藜芦醇单独及联合用药时对人肝癌细胞HepG-2的活性影响,计算出其抑制率和半抑制率浓度,并通过金氏修正公式进行药效判定.结果发现当紫杉醇和白藜芦醇单独用药时,两者对HepG-2细胞的生长均有较高的抑制作用,在设定的药物浓度范围内,前后两者的抑制率分别达到了45.81%-81.80%和34.43%-93.60%,并且呈现出明显的剂量依赖性.当二者联合作用时,紫杉醇与白藜芦醇分别在在5-20μmol/L以及100-1000μmol/L的浓度范围内呈现出相加效果,且药品的半抑率制浓度均出现不同程度的降低,分别由单独用药时的1.47和122.07μmol/L下降至联合作用时的0.65和42.41μmol/L.据此判断,紫杉醇与白藜芦醇联合作用确实可显著增强药物对HepG-2细胞的抑制作用.二者具有相加抗肿瘤效果.Paclitaxel and resveratrol are two common anti-cancer drugs that have proved effective in clinical trials. In this study, we investigated the independent effect of the two drugs as well as their combined effect on the viability of HepG-2 cells, trying to elucidate the joint effect of the two drugs. The independent and combined effects of the two drugs on HepG-2 at various concentrations were measured by CCK-8 assay; inhibitory rates and IC50 were calculated and efficacies were judged according to the Revised Jin＇s Formula. Both paclitaxel and resveratrol showed strong inhibitory effect on HepG-2 cells and at the tested concentration the inhibitory rates were up to 45.81% - 81.80% and 34.43% -96.30% respectively, showing an obvious dose-dependency. The two drugs showed combined effects when used together at the concentration of 5 -20 umol/L for paclitaxel and 100 - 1000umol/L for resveratrol. And their IC50 were also decreased from 1. 47umol/L and 122.07umol/L when used independently to 0. 65umoL/L and 42.41 umol/L when used together. Our results confirmed that combination use of paclitaxel and resveratrol can significantly enhance their anti-cancer effect, that is to say, the two drugs have synergy effect.

关 键 词：紫杉醇 白藜芦醇 人肝癌细胞HEPG-2 CCK-8 

分 类 号：R151[医药卫生—营养与食品卫生学]