几种提取热带假丝酵母总RNA方法比较  

Comparison of several methods of total RNA extraction for Candida tropicalis

作　　者：龙燕[1] 杨升[2] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]湖北大学生命科学学院,湖北武汉430062

出　　处：《实验技术与管理》2012年第9期48-50,共3页Experimental Technology and Management

摘　　要：选择Trizol法、热酚法、酵母RNA提取试剂盒法3种方法提取热带假丝酵母总RNA,通过对总RNA进行质量浓度和纯度分析,比较不同方法对热带假丝酵母总RNA提取的影响。结果表明:使用酵母RNA提取试剂盒法提取热带假丝酵母总RNA是较为有效且简便的方法。Three methods including Trizol method, hot-phenol method, and Yeast RNA kit method were used for the total RNA extraction from Candida tropicalis. Based on total RNA concentration and purity, these methods were compared for their effects on total RNA extraction from Candida tropicalis. The results show the Yeast RNA kit method is the most effective and convenient method for total RNA extraction from Candida tropicalis.

关 键 词：热带假丝酵母 酵母RNA提取 TRIZOL法 热酚法 

分 类 号：Q522-33[生物学—生物化学]