响应面法优化硫酸软骨素提取的酶解工艺  

Optimization of enzymatic hydrolysis of chondroitin sulfate extraction by response surface methodology

作　　者：陈亚[1] 徐晓燕[1] 

机构地区：[1]江苏省滩涂生物资源与环境保护重点建设实验室江苏省盐城技师学院,江苏盐城224002

出　　处：《中国生化药物杂志》2012年第5期548-551,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：江苏省滩涂生物资源与环境保护重点建设实验室开放课题项目(JLCBE11006)

摘　　要：目的以猪喉软骨为原料,提取硫酸软骨素,优化酶解工艺条件。方法采用碱提-酶解-醇沉的方法提取硫酸软骨素,在单因素试验的基础上,通过响应面分析优化酶解工艺。结果酶解最佳工艺组合为:胰酶浓度1.0%、pH值8.6、酶解温度46℃、酶解时间2.8 h。结论采用上述组合,以氨基葡萄糖含量为指标,氨基葡萄糖含量达25.94%。研究结果具有工业应用价值。Purpose The chondroitin sulfate was isolated from pig laryngeal cartilage. The technique of enzymatic hydrolysis of chondroitin sulfate extraction was improved. Methods The chondroitin sulfate was isolated through the process of alkali extraction-enzymatic hydrolysis-alcohol precipitation. Based on the single factor tests,the technique of enzymatic hydrolysis of chondroitin sulfate extraction was improved by response surface methodology. Results To extract the chondroitin sulfate from pig laryngeal cartilage in enzymatic hydrolysis, the best combination of extracting technique was： the enzyme concentration of 1.0% ,8.6 of pH, the temperature of 46 ℃ , and the time of 2. 8 h. Conclusion The content of glu- cosamine in the chondroitin sulfate was 25.94% after the optimization of the technique. The results can be calculated for industrial use.

关 键 词：硫酸软骨素 提取 酶解 响应面法 猪喉软骨 

分 类 号：R282.74[医药卫生—中药学] TQ464.1[医药卫生—中医学]