雷公藤内酯醇抑制CXCL11表达对大鼠重症急性胰腺炎急性肺损伤的影响  

The effect of Triptolide by Inhabiting Chemokine CXCL11 in Acute lung injury of Severe Acute Pancreatitis

作　　者：陈海生[1] 张海峰[2] 陈海琴[2] 曹维[2] 周国雄[2] 

机构地区：[1]南通大学附属海安医院,江苏226600 [2]南通大学附属医院消化内科

出　　处：《交通医学》2012年第3期209-212,共4页Medical Journal of Communications

基　　金：南通市社会发展基金资助项目(S5054)

摘　　要：目的:通过制作大鼠重症急性胰腺炎(SAP)模型,并给予雷公藤内酯醇干预,检测不同时间点肺组织中趋化因子CXCL11的动态变化,探讨雷公藤内酯醇对SAP急性肺功能损伤的治疗作用。方法:72只SD大鼠随机分为3组:假手术组(N组),SAP组(P组),雷公藤内酯醇治疗组(T),每组24只。4%牛黄胆酸钠逆行胰胆管注射建立SAP大鼠模型,剂量为1mL/kg,N组打开腹腔后仅仅翻动胰腺组织数次,T组在造模成功后立即腹腔注射雷公藤内酯醇(100μg/mL)。每组随机分为4个亚组,每个亚组6只。4个亚组分别在1、3、6、12小时抽血、处死后留取组织标本。分别检测各不同时间点组的血清淀粉酶,肺湿干重比,肺组织病理,免疫组化法检测肺CXCL11表达,酶联免疫吸附试验检测血清中的CXCL11的水平。结果:(1)血清淀粉酶值:P组与T组各亚组血清AMS明显升高,与N组比较有统计学意义(P<0.01),T组6h、12h亚组AMS值较P组显著减少(P<0.05)。(2)肺湿干重比值:P组3h、6h、12h亚组,T组6h、12h亚组较N组明显升高(P<0.05),T组3h、6h、12h亚组较P组升高减轻(P<0.05)。(3)肺组织病理与组化:3h、6h、12h P组与T组肺组织损伤明显,其中6h、12h T组较P组损伤减轻。CXCL11免疫组化,T组较P组表达明显减轻。(4)ELISA测定:1h、3h、6h P组CXCL11蛋白较N组与T组明显增高,12h P组与N组和T组比较无明显差异。结论:TL可能通过抑制CXCL11表达及抗炎作用减轻AP急性肺功能损伤。Objective: Severe acute pancreatitis(SAP) models of rats were set up to investigate the exspression of CXCL11 in the lung.The effects of triptolide were evaluated by inhabiting the expression of CXCL11 in AP.Methods:Seventy-Two SD rats were randomly divided into three groups: the control group(C group),the AP model group(P group),and the Triptolide treatment group(T group).and there were 24 rats in each group.SAP group was established by 1ml/kg retrograde infusion of 4% sodium taurocholate into the bili-pancreas duct in SD rats.N group was not injected with 4% sodium taurocholate.In T group,triptolide was injected into the abdominal cavity immediately after the SAP model was achieved.each group was randomly divided into four sub-groups and each sub-group contained 6 rats.The rats were randomly selected to be executed at 1,3,6 and 12 hours later in each group.Serum was amylased,and the wet/dry ratio of the lung and the pathological changes of the lung were measured at different time-point in each group.Protein in the lung was decected by immunohistochemistry.Expression of CXCL11 protein in serum was measured by enzyme-linked immunoadsorbent assay.Results:(1)Serum amylase:serum amylase was significantly higher in P group and Tgroup than in N group at each time point(P<0.01).And amylase of 6h and 12h subgroups in T group was lower than in P group.(2)Lung wet/dry weight ratios:they were significantly higher at 3h,6h and 12h in P group and at 6h and 12h in T group compared with N group.(3)Immunohistrochemistry: Damages of lung tissue at 3h,6h and 12h of P group and T group were significantly higher while at 6h and 12h the damage of T group was slighter compared with P group.T group was slightly lower than P group in expression of CXCL11.(4)ELISA:serum CXCL11 at 1h,3h and 6h in P group was higher than in N group and T group and at 12h no significant difference ocurred when P group was compared with N group and T group.Conclusions:Triptolide may ameliorate acute lung injury by inhibiting the activity of CXCL11 and anti-

关 键 词：重症急性胰腺炎 干扰素诱导T细胞趋化因子 雷公藤内酯醇 急性肺损伤 免疫组织化学法 酶联免疫吸附试验 大鼠 

分 类 号：R576[医药卫生—消化系统]