大黄鱼仔稚鱼不同发育阶段矢耳石形态发育和微结构特征  

Morphological development and microstructure of sagittal otolith of large yellow croaker,Larimichthys crocea during larval and early juvenile stages

作　　者：刘志远[1,2] 李圣法[1] 徐献明[3] 张翼[1,2] 黎雨轩[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,农业部东海与远洋渔业资源开发利用重点实验室,上海200090 [2]上海海洋大学海洋科学学院,上海201306 [3]江苏省海洋水产研究所,江苏南通226007

出　　处：《中国水产科学》2012年第5期863-871,共9页Journal of Fishery Sciences of China

基　　金：国家自然科学基金青年基金(31101901);中央级公益性科研院所基本科研业务费专项资助项目(2011M11)

摘　　要：对人工培育大黄鱼(Larimichthys crocea)的生长发育与矢耳石形态及微结构特征进行研究,结果表明:(1)大黄鱼矢耳石上的轮纹是每日形成的,第1日轮在孵化后第2天形成,与其初次摄食相对应。(2)大黄鱼卵黄囊期和前弯曲期仔鱼的耳石形态为圆形,进入弯曲期耳石长轴迅速伸长,在后弯曲期耳石形态变为椭圆形。进入稚鱼期,矢耳石开始形成次生核。随后次生核数量逐渐增加,在孵化后47～78 d的个体中,次生核数量稳定在5～7个,耳石近似盾形。(3)根据耳石日轮宽度推算的大黄鱼稚鱼在其仔鱼期生长率(b)与第1个次生核的形成时间(tsp1)之间存在明显的线性关系,表明生长较快的个体形成次生核的时间较早,进入稚鱼期所需的时间更短。以上结论表明,大黄鱼矢耳石可以反演其早期生活史阶段的生长发育特征。We documented the morphological development and microstructure of the sagittal otolith during the larval and early juvenile stages （between hatch and 78 d after hatch） of large yellow croaker （Larimichthys crocea） We observed daily periodicity in growth increment formation in the sagittal otoliths. The first daily increment was formed 2 days after hatching （dah）, corresponding to the time of first feeding. The sagittas were round in shape during the yolk-sac larval and pre-flexion larval stages, subsequently becoming elongated on the long axis during the flexion larval stage. Following this, the sagitta became ellipsoid in shape during the post-flexion larval stage. During the early juvenile stage, the first secondary primordium began to form between 29-39 dah （32±0.39）, corresponding to the timing of metamorphosis from larvae to juvenile. The number of secondary primordium ranged from 5 to 7 in 47-78-day-old juveniles, and the secondary growth zone （SGZ） surrounding the first growth zone （FGZ） formed an ＂enclosed structure＂. During this stage, the sagittas were nearly peltate in shape. The radius （R） of the sagitta was significantly and linearly related to standard length （Ls） for all individuals （R =-155.8±39.1 Ls） （n=211, P〈0.001）. The growth rates during the larvae stage （b）, calculated based on the width of the daily incre- ment on the sagitta of juveniles, were significantly and linearly related to the time of formation of the first secon- dary primordium （tsl,1） （b =38.6-161.9tsel）（n=42, P〈0.01）. This suggests that the secondary primordium was formed much earlier and metamorphosis was more rapid in faster growing larvae. In summary, the pattern of early life history growth and development of large yellow croaker can be back-calculated based on sagitta size, morphology, and microstructure. Our results provide a theoretical basis for measuring early growth and development and documenting recruitment of large yellow croaker in the field.

关 键 词：大黄鱼 耳石 日轮 次生核 生长率 

分 类 号：S931[农业科学—渔业资源]